| To explore the effect of Chinese herbal medicine on cock semen quality andfreezing effect. The test select27forty-week-old breeder cocks,via semen collectiontraining and the test of semen quality,select9cocks for groupâ… (the group needs toimprove),which have lower success rate of semen collection,higher pollution rate ofsemen,less semen volume and thinner semen,the other18cocks were randomlydivided intoâ…¡, â…¢. Groupâ… ,â…¡ fed with basic diets supplemented with2%differentChinese herbal medicine, groupâ…¢ fed with basic diets.The experiment results show:1) The text of success rate of semen collection: When test period60d, thesuccess rate of semen collection in â… ,â…¡group increased by38.71%and16.22%than the end of the advanced period respectively (P<0.05), by the13.16%than thecontrol group (P<0.05); drug discontinuance10d, the success rate of semen collectionin â… ,â…¡group increased by25.81%and10.81%than the end of the advanced periodrespectively (P<0.05), by the5.4%and10.81%than the control group (P<0.05).2)The text of pollution rate of semen: When test period60d,the pollution rate ofsemen in â… ,â…¡group reduced by38.2%and42.11%than the end of the advancedperiod respectively (P<0.01), by the37.85%and56.5%than the control group(P<0.01); drug discontinuance10d, the pollution rate of semen in â… ,â…¡group reducedby77.53%and69.92%than the end of the advanced period respectively (P<0.01), bythe66.67%than the control group (P<0.01).3) The rule determine text of qulity of semen: When test period60d,the semenvolume in â… ,â…¡group increased by20.88%and16.41%than the end of the advancedperiod respectively (P>0.05),â…¡group by the11.19%than the control group (P>0.05);the semen motility rate in â… ,â…¡group increased by2.97%and5.03%than the end ofthe advanced period respectively (P<0.05), by the1.88%and3.92%than the controlgroup (P<0.05); the semen concentration in â… , â…¡group increasedby11.09%and5.72%than the end of the advanced period respectively(p<0.01),â…¡group by the2.45%than the control group (P<0.05); the effective sperm number in â… ,â…¡group increased by50.96%and15.38%than the end of the advancedperiod respectively (p<0.01),â…¡group by the10.25%than the control group (P<0.01);the semen abnormal rate in â… ,â…¡group reduced by14.74%and14.08%than the end ofthe advanced period respectively (p<0.01), by the18.77%and13.45%than the controlgroup (P<0.01). drug discontinuance10d, the semen volume in â… ,â…¡group increasedby36.26%and4.69%than the end of the advanced period respectively(P>0.05),â…¡group by the0.75%than the control group (P>0.05); the semen motilityrate in â… ,â…¡group increased by1.07%and2.97%than the end of the advancedperiod respectively (P<0.05), by the4.17%and6.13%than the control group (P<0.05);the semen concentration in â… ,â…¡group increased by5.56%and3.91%than the end ofthe advanced period respectively (p<0.01),â…¡group by the5.73%than the controlgroup (P<0.05); the effective sperm number in â… , â…¡group increasedby47.63%and14.05%han the end of the advanced period respectively(p<0.01),â…¡group by the13.53%than the control group (P<0.01), by the14.86%thanthe number to match the hen; the semen abnormal rate in â… ,â…¡group reducedby19.02%and17.70%than the end of the advanced period respectively (p<0.01), bythe16.58%and16.98%than the control group (P<0.01).4) The text of the semen freezing tolerance: When test period60d,the semenmotility after freezing in â… ,â…¡group increased by17.52%and28.42%than the end ofthe advanced period respectively (P<0.01),by the13.99%and30.98%than the controlgroup (P<0.01); the semen abnormal rate after freezing in â… ,â…¡group reducedby10.81%and6.11%than the end of the advanced period respectively (p<0.01), bythe7.51%and5.30%than the control group (P<0.01);the semen recovery rate in â… ,â…¡group increased by14.13%and22.38%than the end of the advanced periodrespectively (p<0.01), by the11.89%and26.05%than the control group (P<0.01); thesperm motility rate-shape index in â… ,â…¡group increased by7.7%and10.93%than theend of the advanced period respectively (p<0.05), by the6.04%and11.51%than thecontrol group (P<0.05); the sperm effective survival time in â… ,â…¡group increasedby41.03%and48.54%than the end of the advanced period respectively (p<0.01), by the23.21%and52.54%than the control group (P<0.01). drug discontinuance10d,thesemen motility after freezing in â… ,â…¡group increased by16.28%and26.35%than theend of the advanced period respectively (P<0.01),by the11.9%and27.86%than thecontrol group (P<0.01); the semen abnormal rate after freezing in â… ,â…¡groupreduced by7.95%and4.66%than the end of the advanced period respectively (p<0.01), bythe6.25%and5.0%han the control group (P<0.01);the semen recovery rate in â… ,â…¡group increased by15.06%and22.72%than the end of the advanced period respectively (p<0.01), bythe7.44%and20.48%than the control group (P<0.01); the sperm motility rate-shapeindex in â… ,â…¡group increased by6.89%and9.96%than the end of the advanced period respectively (p<0.05), bythe5.17%and10.45%than the control group (P<0.05); the sperm effective survivaltime in â… ,â…¡group as2.08times and2.38times than the end of the advanced periodrespectively (p<0.01),as the1.84times and2.46times than the control group (P<0.01).5) The determine text of semen LDH: Before freezing,the sperm's LDH activityin Chinese medicine group's increased by5.26%than the control group(P<0.05),seminal plasma LDH activity in Chinese medicine group's reduced by1.94%thanthe control group(P<0.05). After freezing,the sperm's LDH activity in Chinesemedicine group's increased by33.67%than the control group(P<0.05), seminalplasma LDH activity in Chinese medicine group's reduced by23.46%than thecontrol group(P<0.01).Experiment Indicated: Chinese herbal medicine may effective improve cock's semenquality and the semen freezing tolerance,thus improve the cock's reproductiveperformance. |
