Study On Single Cell Protein Production With Solid State Fermentation Of Apple Pomace By The Mixed Strains Of Space Mutagenesis

The lack of protein resources is very serious in our country. The 70% of it are dependent on imports. We need to import 35 million tons of soybean,1.2 million tons of fish meal and other protein resources every year. The value of it has been valued at 150 billion RMB. On the other hand, the apple cultivation scale and it's production in China take up 1 / 3 level of the world's production, millions tons of pomace are generated every year . Therefore, the research and development of apple pomace feed SCP (single cell protein), this experiment not only solve the problem of using this huge resources, but also can develop the alternative products for the serious lack of protein resources in our country. In this test, the strains of space mutagenesis, mutagenic strains and other strains are used in producing SCP from fermented apple pomace. The results are obtained as follows:In this test, through the activation of strains after activation 10 groups strains are inoculated on the target material gradient and conducted on the 11 successive generations directional adaptive cultivation, screening and screened out the superiority of strains respectively in the end.The single strain of space mutagenesis inoculation quantity in solid-state fermentation apple pomace SCP, the optimal medium was as follows: Aspergillus niger ZM-8 was 2.0%; Sac.cerevisiae YB-6 was 6.0%. The double strains of space mutagenesis mixed with Aspergillus niger ZM-8 and Sac.cerevisiae YB-6 more effective than single strain fermentation;The ideal conditions of the double strains of space mutagenesis solid state fermentation of apple pomace to produce SCP was: Inoculation ratio (Sac.cerevisiae YB-6 / Aspergillus niger ZM-8) was 3:1, inoculation quantity was 8.0%in total, the temperature was 30℃,the pH was about 5.0 or nature, the fermentation thickness was 4.0cm,and the fermentation time was 3～4d. The fermentation mutant strains with other mixture strains have an advantage over double strains fermentation , The ideal conditions of mutant strains and other strains mixed solid state fermentation of apple pomace to produce SCP was: The optimal mixture ratio of strains and other strains mutation: Aspergillus niger ZM-8: 0.5g,Sac.cerevisiae YB-6: 1.5g, Geotrichum candidum:. 1.0g,Candida tropicalis : 1.5g. The ratio of Aspergillus niger ZM-8﹕Sac.cerevisiae YB-6﹕ Geotrichum candidum﹕Candida tropicalis was 1﹕3﹕2﹕3;The Inoculation was 10.0% in total; the temperature was 30℃, the pH was nature, fermentation time was 4d, material thickness was 4.0 cm.The composition of fermentation raw material and the ratio were determined: the most appropriate ratio of fermentation raw material was : 70% of apple pomace; 10% of corn stalks; 15% of bran; 5% of accessories.The composition of fermentation subsidiary material and the ratio determined: the primary and secondary sequence that the subsidiary material effect on the crude protein content of fermentation products was : Urea> salt>ammonium sulfate; urea: 2g, ammonium sulphate: 2g, salt: 1g. this is ideal materials for the composition and proportion.