| The essay adopts branches and leaves of Chinese H. rham noides as an experimental object and uses enzyme inhibitor's screening model to make AGI extraction of the active substance, select AGI position of high activity. It also makes the external kinetic study of enzymology, the model study of rats'internal sugar tolerance and the test research of DM rats. The aim is to explore the effect of AGI position upon high activity and its functional organism. Now the main contents and results of this research are as follows:1. Obtain 3 different extractions: ethyl acetate, methanol, and distilled water by using different solvents like ethyl acetate, methanol, and distilled water to get three different extractions from the rough powder. Then, polysaccharide substances are drawn by using alcohol reflux hot water extraction method and Sevage to purify polysaccharide. The rate of extractions from ethyl acetate, methanol, distilled water and polysaccharide is respectively 1.52, 1.71, 2.41 and 2.79%.2. Use screening model of AGI to respectively screen the AGI activity of different extractions. The result: the inhibiting activity of ethyl acetate, methanol, distilled water and polysaccharide is respectively 20.34%, 13.43%, 71.28% and 90.43%.3. Select polysaccharide extractions of high activity, and get the sugar content of 28.52%. Make study of the physicochemical property and the external kinetic of enzymology of purified polysaccharide, and result is when the viscosity of polysaccharide is between 0.0098mg/mL-0.625mg/mL, the inhibiting rate of enzyme goes up with the increase of viscosity; IC50=0.01912mg/mL, when the viscosity is 0.625mg/mL, the inhibiting rate is 92.52%. We get the result that polysaccharide is a high active non-competitive AGI by its instant colliquefaction with enzyme, extensive acid base range and good thermal stability. Its inhibiting index Ki = 5.26mg/mL.4. Adopt normal rats'sugar tolerance model and use polysaccharide (purified) as test medicine to observe polysaccharide'effect upon the normal rats with the loading dose of sucrose, glucose, maltose and starch. The result is compared with the control group, sucrose group show the obvious difference (P<0.05), glucose group and starch group show move obvious difference (P<0.01). The maltose and starch group show an obvious reduction of blood value, and next is sucrose group. We observe polysaccharide can increase the sugar tolerance of the normal rats and polysaccharide functions by inhibiting intestinal AG, whose sugar reduction is similar to acarbose's.5. Adopt alloxan diabetic rat model to observe polysaccharide's effect upon high blood sugar rats. The result is that compared with the control group, among polysaccharide high dose group, the blood sugar value of rats, after 2 and 4 weeks'administration, shows this group has an obvious effect upon sugar reduction of high blood rats (p<0.01, p<0.05), with the sugar reduction rate of 31.72% and 45.70% respectively. Only next to this group is the polysaccharide low dose group (p<0.05, p<0.05), with the sugar reduction rate of 28.02% and 39.38%. This shows that polysaccharide has a good effect upon hypoglycemic rats, modeled by alloxan, showing the relevance to intensity and dose of itself.6. Adopt STZ diabetic rat model to observe polysaccharide's effect upon high blood sugar rats. The result is that compared with the control group, the polysaccharide high dose group, after 2 and 4 weeks'administration, shows an obvious effect upon sugar reduction of high blood rats (p<0.01, p<0.05), with the sugar reduction rate of 33.14% and 43.78% respectively. Only next to this group is the polysaccharide low dose group (p<0.05, p<0.05), with the sugar reduction rate of 28.07% and 36.43%. This shows that polysaccharide has a good effect upon hypoglycemic rats, modeled by STZ, showing the relevance to intensity and dose of itself. |
PDF Full Text Request