Extraction,purification And Constituent Identification Of Polyphenols From Areca Nut

Areca nut is the seed of Areca Catechu L witch belongs to palmae. It was widely planted in Hainan, Fujian and Yunnan province; some was planted in Guangxi provin-ce. There are about15%polyphenols in Areca Catechu L and mainly distributed in ro-ot, leaves, flower and seed. The fresh areca nut contains higher polyphenols. The pol-yphenols in areca nut has the effect of antioxi-dant, bactriostasis and antiaging as the functional substance of areca nut. Deeping processing and protective foods of areca nut were the important aspect of areca industrial so that it was important to study on the polyphenols of areca in order to develop health food of areca nut and improve the functional ability of areca. First of all the polyphenols of areca nut was extracted by solvent and the extraction conditions were optimized in this paper; second the crude polyphenols extracted by solvent was purified by macroporous resin Adsorption and the conditions were optimized; third the constituent of polyphenols witch was purified by macroporous resin was identified by HPLC-MS. All the research above were aimed to provide the theoretical basis of developing high value-added product of areca nut and promoted the functions of areca nut.(1) The extraction of polyphenols from areca nut and the optimized of the conditions:the polyphenols of areca nut was obtained by solvent extraction; the methanol was confirmed to be the best solvent with the extraction rate of15.82mg/g.The effect of extraction temperature,extraction time,concentration of methanol and ratio of solid and liquid on the extraction rate was studied through the Single Factor Experiment. The conditions of extraction process were optimized by the Response Surface Method on the basis of above results. The result showed that the optimum conditions of extraction were temperature62℃, time53min, ratio of solid and liquid1:16concentration of methanol86.2%with the extraction rate of17.82mg/g.(2) The purification of crude polyphenols of areca nut: Macroporous Resin Adsorption was adopted to purify the polyphenols of areca nut and the S-8macroporous resin was confirmed to have the best effect through the static adsorption and elution. The adsorption amount and elution amount were14.01mg/g and28.44mg/g respectively. The effect of pH,volume of polyphenois and concentration of polyphenols on the adsorption effect of S-8resin was studied by the dynamic adsorption test and the adsorption conditions was optimized by the Response Surface Method. The result showed that the best adsorption conditions were:concentration of polyphenols2.23mg/mL, volume of polyphenols6.6BV and pH2.2with the adsorption rate of93.062%. Similarly the effect of volume of elution, pH of elution and concentration of elution on the elution effect was studied and the elution conditions were optimized by the Response Surface Method. The result showed that the best elution conditions were:elution volume6.5BV, elution concentration77.4%, eluton pH3.8and the elution rate was92.52%.(3) The constituent determination of polyphenols of areca nut:the HPLC-MS was adopted to identify the component of areca nut polyphenols and the result showed that it contains rutin, chlorogenic acid, gallic acid and catechin. The (m/z) were609.2,353.5,169.land291.5respectively.