The Method For The Determination Of β₂-agonists Residues In Foodstuff Of Animal Origin By HPLC-MS/MS

The method for the determination ofβ2-agonists residues in liver and meat of pig by HPLC/MS-MS (High Performance Liquid Chromatography-Tandem Mass Spectrometry) has been developed.All of P 2-agonists in this project are salbutamol, terbutaline, cimaterol, cimbuterol, ractompamine, clenbuterol, bromobuterol, isoxsuprine, mabuterol, mapenterol, bromclenbuterol.The sample was extracted in acetate buffer of pH 5.2 and extraction was hydrolyzed byβ-Glucuronidase/aryl sulfatase. Then the protein was precipitated with HCIO4. The supernatant was passed through a SCX cation exchange column (MCX,3cc,60mg), Clenbuterol-D9 & Salbutamol-D3was used as an internal standard, and then elevenβ2-agonists can be determined at one time.The experimental results from spiked liver sample indicated that the average recoveries of external ranged from 31.26%-79.88% and the recoveries ranged from 89.4%-110.5% and the RSD were 1.1%-2.8%. The limits of detection (LODs) were 0.5ng/g.