| Water chestnut, also known as water chestnut, water chestnut fact, the door is angiosperms, dicotyledonous plants Gang, myrtle head, Hishina (Trapaceae), is a herbaceous aquatic plants Ling fruit in China has 2,000 years of cultural history. U.S. meat and water chestnut crispy, cooked food can be used for porridge food. Water chestnut is rich in protein, carbohydrates, unsaturated fatty acids, vitamins and trace elements. Our pharmacy monograph, "Compendium of Materia Medica" records: water chestnut can "fill the stomach, strong stock knee, safety in the five internal organs, Dan Stone toxic solution, fill in the sickness, Jian Li Qi." Another study shows that water chestnut contains anti-cancer activity of some chemical substances, such as some of the creatures, etc., but on water chestnut polysaccharides is far from rare, and mostly limited to conventional extraction methods.This paper as raw material to plant water chestnut, use of ultrasonic water extraction, alcohol precipitation of the polysaccharide extracted from water chestnut, and the use of response surface analysis of polysaccharide extraction conditions were optimized; Secondly, water chestnut water chestnut polysaccharide polysaccharide were prepared based oral drug solution, a preliminary study of its stability, and to regulate water chestnut polysaccharide immune function and antioxidant activity of both a preliminary pharmacodynamic study, the same time, the safety of the agents was evaluated.Extract part of the single factor experiments and response surface methods to determine the optimal water chestnut polysaccharide ultrasonic extraction conditions, that the extraction temperature is 52.4℃, extraction time is 36.1min, ultrasonic power is 86.9W, liquid feed ratio is 27.8ml.g -1, optimizing polysaccharide yield 0.1611 percent. Validation experiments show that the optimal extraction conditions of the water chestnut polysaccharide obtained under the actual yield 0.1609 percent, and the response surface model prediction compared to the relative error is -0.124%This paper has been optimized according to conditions of preparation of water chestnut extract polysaccharide, polysaccharide-based medicine to the preparation of water chestnut polysaccharide, and its preparation process has been studied, experimental studies of each water chestnut polysaccharide polysaccharide content 6mg/mL , 10mL / sticks, brown bottle filling and sealing; In addition, this chapter also experiment with clarity, water chestnut polysaccharide content and preservative effects of several of the indexes was investigated by adding different types and amount of additives, preservatives experimental results to determine of sodium benzoate, added at 0.2%; PVP as stabilizer, adding the amount of 0.2%; pH value between the range of 5.0-5.5. Polysaccharide obtained was clarity of the water chestnut yellow, sweet, taste good, anti-corrosion effective, easy to longer-term storage.The paper on the water chestnut polysaccharide regulating immunity and antioxidant two aspects of efficacy studies, experimental results show that different doses of water chestnut polysaccharide can significantly improve immune function in mice serum hemolysin levels, NK cell activity, etc.; in addition, 300mg/kg/d dose of water chestnut polysaccharide not only is immunocompromised mice above parameters returned to normal levels, and can make immunocompromised mice serum hemolysin levels and spleen lymphocyte proliferation was significantly higher than the level of normal mice.Antioxidant activity, the water chestnut polysaccharide on immune suppression in mice have better scavenging free radicals. Experimental data show that water chestnut polysaccharide can significantly increase the immune dysfunction induced by cyclophosphamide in mice AOC, T-SOD and GSH levels, and can significantly reduce the immunocompromised mice, MDA content, and to the above indicators to reach normal levels.In addition, the paper also for the safety of water chestnut polysaccharide was related to evaluation of acute toxicity and subacute toxicity experiments showed that the dose of the water chestnut polysaccharide in mice organs, so there was no significant effect on body weight, all the mice were normal physiological, mental status, feeding the living conditions of normal water intake, no poisoning or death phenomenon.Meanwhile, this paper tests and accelerated by the impact factor test examines the stability of polysaccharide water chestnut, water chestnut polysaccharide to the character, clarity, pH, and polysaccharide content as an indicator, showed that light on the water chestnut polysaccharide slight effect, will test the polysaccharide content decreased, therefore, should be brown bottle filling agents, from light, these measures can be effective to some extent, to avoid the light of the impact test, improve the stability of water chestnut polysaccharide; In addition, the temperature stability of the water chestnut polysaccharide had no significant effect can not be considered. Accelerated test results show that the water chestnut polysaccharide traits and pH with the time change was not significant; the same time, biological test results showed that bacteria, fungi, and the number of E. coli were within the required limits for the test within 6 months of stability in good condition, the water chestnut polysaccharide can thus shelf life of 2 years. |
