HS-SPME-GC Fingerprint Of The Volatile Compounds In Magnoliae Officinalis Cortex

Cortex Magnoliae officinalis for dessicated skin of the tree trunk, branches and the root of magnoliaceous species, Magnolia officinalis Rehd. Ge et Wils and Magnolia officinalis Rehd.et Wils.var. biloba Rehd.et Wils.This topic's object of study is Cortex Magnoliae officinanlis. Cortex Magnoliae officinalis of 15 different production fields are gathered and bought.Use top empty Solid-phase microextraction-gas chromatography method to determine and analyze compositions of volatile compounds in Cortex Magnoliae officinalis of different origins,furthermore incorporating GC-MS evaluation results,a fingerprint of volatile Chinese medicine is established,through results achieved from cluster analysis and similarity analysis evaluation of the quality of the quality of the herbs and at the same time to look for the difference of volatility and it's relevant relation with presence of phenolic compounds in different herbs of different origins, we adopt HPLC method to find out and compare the phenolic composition of the herbs from different production fields; for scientific control of the quality control of Cortex Magnoliae officinalis we evaluate and judge by a systematic approach.Firstly, HS-SPME-GC method is established to analyze composition of volatile compounds and it's extraction condition and optimization by chromatographic process. Through investigation of the number of peaks peak under different conditions and total superficial peak area change differences. For commercial extraction of differently coated SPME,extraction temperature,extraction time and sample usage level are influencing factors in optimization process.Result indication-adopt PDMS/DVB extraction at the beginning,take 0.2g of magnolia bark powder and extraction done under conditions of temperature 70℃and for 60min achieves efficient extraction comparatively. Also on chromatographic column, warming procedures, velocity, diversion ratio separation etc, factors for the optimization conditions, Screened HP-50 capillary column (30 m*0.32 mm*0.25μm),temperature programming-initial temperature 50℃(held for 1min),increased by 4℃/min to 140℃(held for 2 min),increased 2℃/min to 180℃, increased 12℃/min to 280℃(held for 5 min),ate of flow 1.3 ml/min, split ratio of 15:1 for chromatographic condition is suitable for analysis of volatile organic compounds magnolia bark.Secondly, In accordance with the above optimization conditions for batch analytical procedure of bark of Magnoliae officinalis of 15 different origin,altogether obtained 42 common GC peaks,thereby establishing fingerprint,through cluster analysis,those 15 herbs were categorized into 4 different classes,among which classes 1 and 2 are recommended products, choosing 10 Cortex Magnoliae officinalis of different origins,established common mode,furthermore the similarity of comparative illustrations of the calculation produced with the group of 15 herbs. Result indication-similarity even above 0.9, furthermore adopting GC-MS for determining volatile composition in Magnoliae officinalis bark is analyzed; commonly identified 15 constituents among which content ofα-Eucalyptus leaves oleyl alcohol andβ-Eucalyptus leaves oleyl alcohol was highest.Thirdly, For major efficacy, phenol composition,comparative analysis process of 21 batch herbs of magnolia officinalis,it established that it has a common pattern between them,according to honokiol and honokiol investigated indication,use systematic cluster analysis process for identification research of the chemical pattern,22 batches of medicinal material categorized into 4 classes,among which 1 class is the recommended product sample,better reflection of the fact that different origins of magnolia officinalis have different phenolic composition,be it magnolol or honokiol,furthermore with GC method a fingerprint was established related to the composition of volatile compounds of magnolia officinalis and it's analyzed,researching GC fingerprint and it's analytical results and rather than determining relationship between effective analysis content difference,trials through GC and HPLC, two different chromatographic analytical methods can systematically evaluate quality of the herb.In reality allowing fingerprint to reflect the the inherent quality of Chinese medicine.