Study Of Expression Of Mouse Klhdc10 Gene During Embryonic Development

Mouse Klhdc10 gene belongs to a member of the Kelch domain containing proteins superfamily of which many members have been well studied. Many members are proved to participate in some physiological processes and are important for tissue morphogenesis during embryonic development. However, so far there are not any studies about Klhdc10. In this study, we characterized the expression of Klhdc10 by semiquantitative RT-PCR, in situ hybridization and real-time quantitative RT-PCR (qRT-PCR) in order to reveal Klhdc10 expression patterns during mouse embryonic development and make basis for further function study.Bioinformation suggested that the sequence similarity between mouse Klhdc10 and human KLHDC10 protein is 96.61%. Klhdc10 and KLHDC10 have two highly conserved Kelch motifs, respectively. Semi-quantity RT-PCR indicated that Klhdc10 is widely expressed from embryonic day 9.5 (E9.5) to E18.5 with the highest expression at E11.5. In situ hybridization indicated that Klhdc10 signals are prominent in craniofacial regions, especially in telencephalon and optic vesicle at E9.5. Klhdc10 signals were obviously detected in the telencephalon, diencephalon, mesencephalon and rhombencephalon at E10.5 and E11.5. Abundant signals were observed in brain, tongue and muscle of back at E12.5 and E15.5. Moreover, at E15.5, there are also stronger signals in liver and kidney. QRT-PCR results indicated that Klhdc10 mRNA was highly expressed in brain at E12.5, E15.5 and E18.5; while constantly lower in lung and heart. High expression levels were also detected in tongue and kidney (E12.5, E15.5) and liver (E18.5). Further studies suggested that Klhdc10 was widely expressed in the brain such as neocortex, cerebellum and hippocampus at E12.5, E15.5 and postnatal day 0 (P0); however, Klhdc10 signal was mainly confined to cerebellum at P7. Klhdc10 mRNA expression in the brain was increasing as development progressing and peaked at E15.5, while decreased thereafter but constantly maintaining relatively high expression until to birth.In placentas, Klhdc10 signals were abundantly detected in labyrinthine layer at E10.5 and E12.5, while mainly located in spongiotrophoblast layer at E15.5, E16.5 and E18.5. Klhdc10 mRNA was low from E11.5 to E15.5 and was highly expressed at E16.5, maintaining high expression level thereafter. Our results suggested that Klhdc10 was widely expressed in embryonic and extraembryonic tissues, exhibiting dynamic expression patterns. Klhdc10 is likely to participate in the morphogenesis of diverse embryonic and extraembryonic tissues, playing potential important roles in mouse embryonic development.