Study On The Correlation Of Ypel3 Expression With Mouse Embryonic Development

In the preliminary study, Ypel3 was found to be differently expressed by through the subtractive hybridization between parthengenetic and normal embryos. Ypel3 locates on mouse chromosome 7qF3 and contains five exons. The full length of Ypel3 mRNA is 1 057 bp. A CpG island containing 56 CpG sites was found at the upstream of Ypel3 gene, which overlay the promoter, exon 1 and part of intron 1. The imprinted status, gene expression and the epigenetic regulation of Ypel3 were analyzed respectively, which lay the foundation for the future study of Ypel3 function.The imprinted status of Ypel3 was analyzed by SNP direct sequencing. The results showed that Ypel3 was bi-allelic expressed in brain on E18.5 in mouse. The temporal specified expression of Ypel3 on E11.5, E13.5 and E15.5 was analyzed by in situ hybridization. The results showed that Ypel3 was mainly expressed at brain and neural tube on E11.5. The signals of Ypel3 were mainly detected in forehead, midhead, hindhead, tongue, lung and liver on E13.5. The expressions of Ypel3 were found at the endodermis of the lateral ventricle, mid ventricle and cerebellum, thalamus and pituitary of brain. Besides, its expression was detected in spinalcord, nasopharynx, tongue, lung, liver and kidney on E15.5. The spatial expression of Ypel3 was further studied in different tissues by quantitative real-time PCR on E15.5. The results indicated that Ypel3 was widely expressed at brain, tongue, heart, lung and liver, with the highest expression at brain. The quantitative PCR of brain showed that the expression of Ypel3 was increasing from E12.5 to E15.5, reaching the highest at E15.5 and decreasing after that. While at kidney, the Ypel3 expression was increasing from E12.5 to E18.5. During the embryo development, the expression of Ypel3 was persistently increasing from E10.5-E18.5 by using quantitative PCR analysis. The epigenetic regulation of Ypel3 was analyzed by treated Neuro-2a (N2a) cells using 5-Aza and 4-PBA. The results indicated that Ypel3 expression had no significant change by treated with 5-Aza, increased by treated with 4-PBA, and further increased by 4-PBA and 5-Aza in combination.These results suggest that Ypel3 may play an important role in the multi-organogenesis of mouse embryonic development, and its expression may be regulated by histone acetylation in mouse N2a cells.