Whole Brain Neurotransmitters And Receptor Content Of Three-dimensional Distribution Modeling And Visualization,

At present, study on brain has gone deep into the cellular and molecular level. However, there still are many intermediate levels from research on cells in micro-system to that on brain function and behavior in the macro-system. In order to further the study of the brain should carry out from multi-level such as the behavior, tissue, cells and signaling molecules.In this paper, we cut the brain into 5mm×5mm×5mm pieces, recording their coordinates, get the core of each small piece and make them into tissue chips. And then we will use immunohistochemistry, in situ hybridization, laser Raman spectroscopy, terahertz technology to detect neurotransmitter, receptor in each small piece (this research will carry out by our cooperative department). Make use of three-dimensional reconstruction to establish brain neurotransmitter, receptor quantitative distribution model in brain, so that it can provide a basis platform for research on the relationship between brain neurotransmitter, receptor quantitative distribution and brain function. At the same time, rebuild the brain from sequences brain-pieces images, in order to provide an accurate reference model.Tissue chips can delivery the three-dimensional brain neurotransmitters, receptors distribution with advantages of low-cost, small error, fast, efficiency. It helps people to further study on other substances, and then on the brain function. Three-dimensional reconstruction technique can process enormous data in high speed, so that people can directly observe the brain neurotransmitters, receptors distribution and facilitate the comprehensive analysis of the relationship between neurotransmitter, receptor quantitative distribution and brain fuction.