Synthesis Of Conductive Polymer And An Enzyme Sensor

1.Immobilizing Enzyme Using Conducting Polymer CompositeMaterialThe preparation and properties of glucose oxidase biosensor using conducting polymer composite material consisted of ethyl cellulose and acetylene black is reported. The final biosensor is constructed by applied the mixture containing 0.010 g ethyl cellulose, 0.004 g acetylene black, a little paraffin wax and 0.2 mg glucose oxidase on a platinum electrode (3 mm×3 mm). Effect of various factors on the response current of the biosensor was discussed. The response current increases linearly with increasing glucose concentration under 11.5 mmol dm~-3. Its activation energies are 40.3 kJ mol~-1 in enzyme-catalyzed reaction. AFM shows that conducting polymer composite material-glucose oxidase electrode's film presents uniform granular structure after paraffin wax is washed with cyclohexane, which is conducive to enzyme-catalyzed reaction.2.Polyaniline-Uricase Biosensor Prepared with Template ProcessPolyaniline (PANI) uricase biosensor prepared with template process is first reported in this paper. The fabrication process is as follows: PANI-uricase electrode is obtained using one-step process. Then the electrode is hydrolyzed in 6.0 mol dm~-3 hydrochloric acid solution to remove the uricase that may be affected by aniline monomer from PANI film. Finally, active uricase is immobilized into the PANI film again and PANI-uricase biosensor is obtained. Some factors that affect response current are studied, such as temperature, pH, potential and substrate concentration. The determination of biosensors indicates that the response current of the biosensor prepared by template process only decreases by about 18% for 60 days, but that prepared bytwo-step process decreases by approximately 39% for 40 hours. The uricase in PANI-uricase biosensor prepared by template process mainly interact with the nitrogen that link with quinoid ring. It is characterized with FTIR and UV-vis for the first time.3. Polyaniline glucose oxidase Biosensor Prepared with TemplateProcessA new method of preparing polyaniline (PANI) glucose oxidase sensor, which is called template process, is reported in this paper. The process is as follows: Firstly, PANI glucose oxidase electrode is obtained using one-step process. Then the electrode is hydrolyzed in 6.0 mol dm"3 hydrochloric acid solution to drop the glucose oxidase affected by aniline monomer out from PANI film. Finally, active glucose oxidase is immobilized into the PANI film again and PANI glucose oxidase sensor is obtained. Some factors that affect response current are studied, such as temperature, pH, potential and substrate concentration. The stability of biosensors indicate that the response current of the biosensor prepared by template process comes to a maximum at the first two days, then decrease only 4.6% for 30 days; but that prepared by two-steps process decreases approximately 55.4%. The experiment show the glucose oxidase in the biosensor prepared by template process mainly locates at the nitrogen of quinoid ring.4. Electrochemical Copolymerization of Aniline and 0-ChloroanilineThe electrochemical copolymerization of aniline and o-chloroaniline using cyclic voltammetry is reported. The copolymerization of aniline and o-chloroaniline was different from the monopolymerization of aniline or o-chloroaniline in growth rate, redox potentials, and the charge transport mechanisms. The copolymer possesses good solubility in common organic solvents, such as NPM, DMF, DMSO or acetone. FTIR spectra and SEM were used to determine the possible structure and the morphology forcopolymer, respectively. According to the image of SEM and the solubility, the copolymer isn't the simple mixture of polyaniline and poly-o-chloroaniline. The electrochemical properties of the copolymer are mainly determined by PANI, but the copolymer has better electrochemical reversibility and solubility.5. Preparation and properties of an uricase biosensor based oncopolymer of o-aminophenol-anilineAn uricase biosensor prepared with a new copolyme...