Microfluidic Chip Control Fluid Dynamic Observation Platforms And Experimental Research

Microfluidic chip is one of the fronts of modern analysis sciences. As an important and fast developing component of uTAS, it will bring us dramatic advances through its promising application in the life science, environment science, medicine science and chemistry. Since Berkeley institute realized high-speed DNA survey in the microfluidic chip in 1995, it have been applied successfully in the detection of amino acid, protein, cells, drugs and trace elements. These applications display the great potential in the coming future.While its development is a short history of tens of years, the chips' basic research on flow mechanism drops far behind its application research. Using as a reference of the domestic and international research interest, this thesis applies flow visualization to achieve the internal parameters of flow in the chips. Through the experiments it is found that the elemental theory of electro-osmotic flow is appropriate in the microfluidic chip except the geometry factors' nonlinear effect.Basing on the characteristics of the mcirofluidic chips manufactured in the MST center of DUT, this thesis discusses the main contradictions and key factors in the implement of visual observation platform. After the calculation and tradition the uPIV experiment system is set up with reasonable specification. The platform is able to do experiments on particle image velocity and laser induced fluorescence. The algorithm of correlation is tested in Matlab. The thesis also makes experiments of Vitamin B2 and fluorescein Sodium.The thesis establishes a resistance equivalent module for intersection chips on the basic of EOF theory and volt-ampere characterization. It helps to analyze the interaction among microchannel's length, width, current, electric strength and EOF velocity. Using the measurement methods of conductance and image visualization, the thesis gains the EOF mobility in borate solution. In order to specify the effect of geometry factor, a chip designed with microchannels of different width is used to experiment in EOF velocity. As a result, it's discovered that the width has a nonlinear effect on the EOF in microfluidic chips. Thereafter, three injection mode: suspending injection, pinched injection and gated injection are examined in double T microfluidic chips as well as their simulation in Conventorware software. Because leakage is common in the tests, the thesis improves the separation mode by adding a process of push sample to prevent sample from disturbing the separation effect. This process is driven by the power supply controlled by compiled software.Through amount of experiments, the thesis draws a common criterion of processing to reduce the influence of minor factors. In the end of the thesis, the further research's direction is forwarded.