| Objective:Insulin resistance has been become an important problem of human health, the prevention and treatment of cardiovascular disease induced by insulin resistance to be the research emphasis of the medical treatment. Because of the main fatty acid oxidation on normal myocardial energy supply, the role of cardiac muscle in regulating the body glucose homeostasis is not obvious. Therefore, compared with the main organ skeletal muscle, liver, adipose tissue, myocardial insulin resistance in glucose metabolism can not afford a major role in a long time and doesn’t attract enough attention. The harm of myocardial insulin resistance becomes the focus of research in recent years. Myostatin (MSTN) was shown to inhibit muscle growth, inhibition of AKT activity such as insulin resistance. Studies have shown that MSTN as myocardial evil fluid transmission work and studies have shown that the lack of MSTN doesn’t cause the change of heart function and cardiac muscle fiber, the physiological effects of myocardial and skeletal muscle showed obvious differences. This study will get through different ways of movement and the joint motion scheme of MSTN antibody intervention ways on rats model with insulin resistance,which studys from biochemical, morphology, protein, the level of gene different angles.Methods:This study bought healthy male SD rats (Sprague-Dawley) 70 (quality qualified number:shan medical card words 08-004),2 months of age, weight 170～170 g, aseptic environment cage, aseptic environment cage, raising the temperature of 22～25℃, humidity 40～60%, free drinking water, adaptability raised after a week, were randomly divided into normal group (NC,10) and a high-fat diet group (HF,60). NC groups acquired from xi’an jiaotong university medicine laboratory animal center aseptic rearing SD rats (Sprague-Dawley), normal group feeding feed purchased from xi’an jiaotong university medicine laboratory animal center, feeding by breeding rodents standard, by feeding high-fat diet group feed to buy butter, egg yolk powder, sugar, etc to the xi’an jiaotong university medicine laboratory animal center, on behalf of processing. Group raised after 3 months, by oral glucose tolerance test (OGTT) and insulin tolerance test (1ST), body weight, abdominal fat, holding power, such as ultrasonic index to evaluate high fat and sugar to induce insulin resistance model after the build is successful, the intensity in the gym, aerobics, MSTN antibodies intervention,climb the ladder of strength,MSTN, strength and aerobic exercise combined with 5 kinds of sports in gym exercise intervention of model group rats, by blood flow dynamics,kinetics of ultrasonic, holding power and other indicators, biochemical indexes such as enzyme-linked immune response;morphological indexes such as HE staining,MASSON staining and immunohistochemical.Molecular techniques for testing such as Western Blot, RT-PCR and others.It is used of analysing that different groups within the rat cardiac changes and the key molecular protein gene expression of the insulin receptor, myocardial MSTN/PI3K7Akt in the signal path.Results:1. The resistance movement and the aerobic exercise and joint research and analysis on the improvement of the insulin resistance of myocardial cellsResistance exercise, aerobics, aerobics resistance movement joint can significantly reduce insulin resistance rats weight and heart weight (P<0.01), increased heart coefficient (P<0.05, P<0.05, P<0.01), decrease the Lee’s index (P<0.01). Ultrasonic detecting results and aerobic exercise combined aerobic and resistance exercise of left heart ejection fraction (EF), left heart shortening fraction (FS) and heart rate (HR) there was no significant influence; The resistance movement significantly improved EF (P<0.01), to the FS, the HR there was no significant change. Hemodynamic detect resistance exercise, aerobic exercise and its combination of HR, left ventricular pressure maximum rate (±dp/dmax) there was no significant change, significantly lower left ventricular end-diastolic pressure (LVEDP) (P<0.01, P<0.01, P<0.05). HE staining, according to the results of three types of intervention can significantly improve the myocardial cells cross sectional diameter (P<0.01). Masson trichromatic dyeing, according to the results of three kinds of exercise can significantly reduce the degree of myocardial fibers surrounding collagenous fibrosis (P<0.01); Resistance movement and resistance and aerobic exercise can significantly improve the perivascular collagen fibrosis degree (P<0.01). Immunohistochemical experiment results show that the three kinds of motion scheme can significantly reduce the amount of MSTN protein expression (P<0.01, P<0.05, P<0.01), but the rt-pcr detect MSTN receptor ActR Ⅱ beta expression quantity highest, and significantly lower than that of NC group and HL group (P<0.05). By Western Blot and PR-PCR to detect changes of insulin resistance rats myocardial, aerobic exercise, resistance movement and the combination of aerobic exercise group increased significantly and quantity of the protein expression of insulin receptor (InsR) mRNA transcription level (P<0.01); A significant increase in PI3K transcription level (P<0.05, P<0.01); The transcription of nucleic acids significantly increased Akt (P<0.01); Have a higher protein expression effect on GLUT4, but there was no significant; Reduce the amount of protein expression GSK3 beta and mRNA transcription level, there was no significant. Resistance exercise can improve the quantity of PI3K protein expression and mRNA transcription, there was no significant change, significantly improve the Akt protein expression; Has the improvement effect on the GLUT4, was not significant; Significantly reduce the amount of GSK protein expression (P<0.05); Three kinds of scheme can significantly reduce the amount of FoxO1 protein expression (P<0.05), mRNA transcription there was no significant change. Resistance movement and the aerobic exercise can significantly improve mTORmRNA transcription level (P<0.01), the joint group had no significant change.2. The resistance movement and MSTN antibody interference and joint research and analysis on the improvement of the insulin resistance of myocardial cellsResistance movement and MSTN antibodies and their combined intervention scheme can significantly reduce insulin resistance in the rat body weight, heart weight (P<0.01, P<0.05), improve heart coefficient (P<0.05), reduce the body length (P<0.05); And the resistance movement and joint group can significantly reduce the Lee’s index (P<0.01). Ultrasound, according to the results of resistance exercise can significantly improve left heart shortening fraction (P<0.01), improve the cardiac systolic function, had no significant effect on heart rate; Antibodies to intervention group and combined group can improve left heart systolic function, but there was no significant; The other antibodies intervention group HR can promote rats (P<0.05). Hemodynamic detect resistance movement and joint plan affect LVEDP have significant difference (P<0.01), antibody of MSTN intervention did not have significant changes on cardiac function. MSTN antibody interference and joint can significantly improve left ventricular pressure maximum rate (±dp/dmax) level. HE staining, according to the results of three intervention scheme can significantly improve the myocardial cell cross sectional diameter (P<0.01). Masson trichromatic dyeing, according to the results of three types of intervention scheme can significantly reduce the myocardial fibers surrounding collagenous fibrosis degree and perivascular collagen fibrosis degree (P<0.01,P<0.05). Immunohistochemical experiment results show that the three intervention scheme can significantly reduce the amount of MSTN protein expression (P<0.01). RT-PCR detection group HL MSTN receptor ActR II beta expression quantity reduced but there was no significant in the HF group. By Western Blot and PR-PCR testing technique for insulin resistance in rats to study the changes of myocardial, three kinds of expression of insulin receptor nucleic acid level intervention plans there was no significant effect, but has a significant effect on protein expression level. Resistance movement increase PI3K protein levels, there was no significant; MSTN antibodies and associated with resistance groups can significantly improve PI3K protein levels (P<0.01, P<0.05). Resistance movement, antibody immune intervention significantly enhances the level of Akt protein expression levels (P<0.05); Resistance movement, antibody of MSTN intervention can significantly improve Akt nucleic acid quantity expression. Higher resistance movement of GLUT4 protein expression and the function of DNA transcription, but there was no significant; Antibody immune intervention can significantly improve the expression level of nucleic acids GLUT4 and quantity of protein expression (P<0.01,P<0.05). Resistance movement, reduce the amount of protein expression GSK3 beta (P<0.05); Three kinds of scheme intervention were significantly lower GSK3 beta DNA transcription. United resistance movement and MSTN antibody significantly reduce the amount of FoxO1 protein expression (P<0.05); Resistance movement, antibody of MSTN intervention there was no significant change. Resistance movement significantly improve mTOR mRNA transcription level (P<0.01), three kinds of scheme intervention significantly improve mTOR quantity of protein expression (P<0.05).Conclusion:1. The resistance movement has attained obvious effect on that aerobic exercise intervents insulin resistance rats myocardial. Aerobic and resistance exercise intervention of insulin resistance rats myocardial signal path are not completely consistent.Aerobic exercise is mainly improving insulin resistance rats cardiac symptoms by improving the IRS-1/PI3K/Akt signaling pathway,then resistance movement gets through MSTN and its receptor and PI3K/Akt, increasing the muscle area, improving insulin resistance. The two signaling pathways are in intersection, but not identical. The effects of two exercises are similar on cardiac function, increasing the myocardial area and the improvement of the inhibition of collagen fibrosis. Resistance exercise has obvious effect on protein expression and cell growth. Aerobic exercise mainly works through reducing the occurrence of sugar dysplasia and promoting the synthesis of glycogen. The effect that aerobic exercise loses weight is most obvious,the joint of aerobic exercise and resistance has no expression of superposition effect.2. The resistance movement and MSTN antibodies are paralleled to improve cardiac function, increase the myocardial area, inhibition of collagen fibrosis.They have same signal transduction mechanism and the joint of both intervention donnot have the stacking effect on myocardial insulin resistance. MSTN antibodies can improve myocardial insulin resistance.It infers that resistance improvs insulin resistance of cardiac function in rats through MSTN pathways. |
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