Turmeric Fried In The Serum Of Rats After Vascular Injury Bcl-2 And Bax And P53 Expression In Experimental Research

Purpose:To investigate the turmeric apoptotic factor Bcl-2 and Bax and the expression of p53. Speculated that vascular endothelial cell damage control mode and traditional Chinese medicine turmeric possible mechanism of apoptosis of endothelial cells, for PCI after"restenosis"to provide experimental evidence for prevention and treatment.Material and method:The simulation result of intimal injury during acute intimal injury, which turmeric group 36, model 36, with 36 sham group and 36 normal group as control. Turmeric sets of the intimal injury in the general routine postoperative turmeric 100mg/Kg/d administered; model sets of the general routine postoperative intimal injury saline 3ml / d orally; sham laparotomy suture not insert the balloon catheter; normal control group did not use any intervention. In the simulation, respectively 3,7,14 days after the first use of light and electron microscopy changes in the structure of rat endothelial cells; detected by ELISA in 144 serum and the Bcl-2 and Bax expression of p53.Results:1. Light microscope, turmeric group 7 days after injury medication 7 days compared with the model group of local endothelial vascular injury and intimal damage off slightly reduced the extent of vascular stenosis can be seen to reduce. And normal control group, but there are still differences in lumen area were significantly smaller than the undamaged side.2. Electron microscope can be seen in the third day after balloon injury irregular columnar endothelial cells, cell shrinkage, cytoplasmic stain, nuclear fragmentation. Shows that the release of a small number of apoptotic bodies. Seventh day, cell necrosis increased significantly. Model group obviously necrotic cells were observed, there are a lot of fat soluble lipid, and necrotic material, while turmeric fried necrotic cells compared with the other control group decreased only slightly, a few apoptotic debris. 3. Bcl-2 and Bax and the expression of p533.1 in the first 3 days after drug intervention, turmeric fried group and model group, sham operation group decreased Bax, Bcl-2 content was increased, P <0. 05; turmeric fried group p53 levels compared with the model group difference P < 0.05, compared with the sham group no significant difference, P> 0. 05. With the normal control group, P> 0. 05.3.2 in the drug intervention day 7, 14 days after the turmeric group and model group, sham operation group decreased Bax, Bcl-2 content was increased; P <0. 05; turmeric fried group p53 content model group, sham surgery group had significant difference P <0. 05. With the normal control group, P> 0. 05.Conclusion:1. Using light and electron microscopy after simulation of vascular balloon injury in rats changes in the structure of endothelial cells, indicating turmeric endothelial protective effect of a relatively clear.2. Chinese medicine turmeric intervention up-regulated Bcl-2 protein expression in rats, so that Bax protein and p53 protein expression was decreased, the ratio increased to Bax/Bc1-2, excessive apoptosis of endothelial cells. The ratio between the two is to determine whether apoptosis of important cell regulatory factors. Turmeric can also block the cell cycle, reducing the expression of P53 protein, the rapid restoration of endothelial function, vascular endothelial cells remained intact.3. Apoptotic factor Bcl-2 and Bax and p53 involved in the PCI patients, "restenosis" in the pathophysiological process.4. Detection of serum Bcl-2 and Bax and p53 content of the abnormal changes that may help to assess apoptosis after PCI factor on vascular endothelial function.