Study On The Effects Of Turmeric Oil On Keratinocyte Proliferation And Differatiation In Vitro

Objective The effects of turmeric oil on keratinocyte proliferation and differentiation in vitro were observed and the scientific basis for the treatment of proliferatory skin diseases was provided. Methods The culture of human transformed keratinocyte cell line COLO-16 cells was used as a model. COLO-16 cells were grown in plastic cell culture bottles and cultures were maintained at 37℃ , 5% CO2, and 100% humidity in complete Roswell Pack Memorial Institute(RPMT) 1640 medium. COLO-16 cells were inoculated in 96-well flat-bottomed plates at a concentration of 5 x lOVml. Turmeric oil was dissolved by a little DMSO and diluted to 5 concentrations by 3%RPMI1640 medium: 10'3, 10"4, 10'5 , 1Q-6, 10"7 . Control group was 0.2% DMSO. Cells growth was observed every day with an inverted microscope. Cell viability and growth were assessed with trypan blue staining (typically>90% viable) and haemocytometer counting, and a tetrazolium-based colorimetric assay(MTT assay). The absorbance values were evaluated at 495nm after COLO-16 cells were treated by turmeric oil 3 days. The effects of turmeric oil on the expression of proliferating cell nuclear antigen (PCNA) were detected by means of immunohistochemical method SABC with monoclonal anti-PCNA antibody and using image pattern analysis technique. Proliferating COLO-16 cells were treated byturmeric oil of different concentrations for 2 or 3 days. The medium was dumped and cell deposition were colleccted. Electron microscope samples were made in routine methods and the exchange of ultrastructure of COLO-16 cells was observed by a transmission electron microscope. Results MIT assay showed that the inhibiting effects of turmeric oil on COLO-16 cells were significant. The inhibiting effect were increased along with the increasing concentrations of turmeric oil. PCNA expression level decreased gradully with the increasing concentrations of turmeric oil and it had significant difference with control group (P<0.01).The nucleus, mitochondrion and cytoskeleton of keratinocytes were directly damaged .Conclusion The results of the study suggest that turmeric oil may provide suppression for the proliferation and differentiation of keratinocytes in vitro.It is proposed turmeric oil may become a new cure for proliferating skin disease.