Pharmacological Research. Bitter Far Capsules

Purpose:The purpose is to investigate the sedation and hypnosis effect of Kuyuan capsule.The topic will provide an experimental evidence for new TCM drugs in treating insomnia which with definite material basis,good efficacy,low toxicity,and controllable quality.Method:This research subject through the special pharmacodynamics tracking test to determine the best prescription and the optimum extraction process. Detect median lethal dose(LD50) and the confidence limit of 95% of Kuyuan capsule by the bliss method. Through the effect of Kuyuan capsule on locomotor activity in mice,the effect on mice hypnosis of pentobarbital sodium of subthreshold dosage,the hypnotic time experiment on mice by pentobarbital sodium in threshold dosage and the analysis of electroencephalogram on rats,we carry out the pharmacodynamic study of Kuyuan capsule with the behavior experiments and electrophysiological techniques.Results:1.Prescription No.2 could enhance the number of asleep mice of pentobarbital sodium in subthreshold hypnotic dosage(P<0.01).Prescription No.1 could shorten substantially the rats' sleep latent period and prolong obviously the rats' sleep time which induced by pentobarbital sodium at threshold dosage(P<0.05). Prescription No.2 was certified as the optimal prescription by using comprehensive scoring method.2.Process No.1 and No.4 drug group could enhance the number of asleep mice of pentobarbital sodium in subthreshold hypnotic dosage(P<0.05), process No.2 drug group could prolong obviously the rats' sleep time which induced by pentobarbital sodium at threshold dosage(P<0.01),process No.4 drug group could shorten substantially the rats' sleep latent period and prolong obviously the rats' sleep time which induced by pentobarbital sodium at threshold dosage (P<0.05). The results showed that process No.4 was the optimal process.3.The median lethal dose(LD50) of Kuyuan capsule was 42.61g/kg.The confidence limit of 95% was 36.97～49.10g/kg.4.The middle dosage and high dosage of Kuyuan capsule could significantly inhibit the locomotor activity in mice(P<0.05).Low dosage, middle dosage and high dosage of Kuyuan capsule could enhance the number of asleep mice of pentobarbital sodium in subthreshold hypnotic dosage(P<0.01). Middle dosage and high dosage of Kuyuan capsule could prolong obviously the rats' sleep time which induced by pentobarbital sodium at threshold dosage(P<0.05).The experimental results indicated that contribution to sleep phase of the normal rats,SWS1 and the percentage of brain wave(δ)(P<0.01)of the normal rats were all significantly prolonged after administration of high dosage of Kuyuan capsule.Conclusion:1.Prescription No.2 was certified as the optimal prescription of Kuyuan capsule.2.Process No.4 was the optimal process of Kuyuan capsule.3.The median lethal dose(LD50) of Kuyuan capsule was 42.61g/kg.The confidence limit of 95% was 36.97～49.10g/kg.4.Pharmacodynamics proves that Kuyuan capsule really has hypnotic and sedative effects.