Acupuncture On Of Asthma Rat Lung And Colorectal SP-A MRNA Expression Regulation

Objective: Through reveal the expression change of lung and large intestine SP-A mRNA gene in physiological and pathological way, initially promulgates the biology mechanism and the connotation of the"lung is connectedwith the large intestine"TCM theory, provides the new research mentality for the Chinese medicine viscera correlation material base research. Simultaneously draws support from the integrity (multi-organ nature)control action of acupuncture. By interior and exterior point take the internal organs outside and inside related research the adjustment method, reveal the acupuncture control action to lung and large intestine relevance being out of balance, enhance the acupunture therapy clinical effect in lung and large intestine pathological change, promotes the lung and the large intestine outside and inside coincides under the theory instruction acupuncture clinical practice and the development.Method: Divides 56 SD mouse into 7 groups by computer stochastically, the model group and the acupuncture group's SD mouse divides using the albumen sensitization and stimulates asthma outbreak; the blank control group mouse does not make any processing, normal raising; Bundle group mouse only bundle not acupuncture. The mouse do bloodletting execution, cuts according to the request takes the lung, the large intestine, the small intestine, the heart, the stomach and so on 5 kind of organizations, and puts on rapidly the ice tray, completes the mark, saves in the supercold refrigerator. Increases the SP-A gene using RT-PCR, to study each group of mouse through the agarose electrophoresis to organize SP-A mRNA expression level differences.Result: (1) After albumen stimulation, asthma model group mouse's airway resistance elevates obviously (p=0.000), the airway resistance reach the peak after the albumen stimulates in 3 minutes, when 1 to 9 minutes with the blank control group, the bundle group and the sham-operation group compares, obvious markup (p<0.05); The bundle group, the sham-operation group and the blank control group compare the non-significance difference (p>0.05); but the blank control group big mouse in 30 minutes in airway resistances does not have basically changes (p=0.999). (2) After acupuncture treatment, asthma acupuncture group mouse airway resistance when 2 to 7 minutes compares asthma model group to drop 77.5% separately, 71.1%, 57.9%, 44.9%, 51.7% and 49.3%;"yuanluo"group mouse airway resistance when the 5th minute compares with asthma model group obviously reduces (p<0.05).(3) The bundle group, the sham-operation group and the blank control group compare, the lung organizes SP-A mRNA the expression exceptionally to elevate; asthma model group big mouse lung organizes SP-A mRNA expression and the sham-operation group compares reduces obviously, has the extremely significance difference (p<0.01).(4) The bundle group large intestine organizes SP-A mRNA expression and the sham-operation group big mouse compares, has the significance difference(p<0.05); The sham-operation group big mouse large intestine organizes SP-A mRNA expression and the bundle group compares, has the significance difference (p<0.05);"sanxuewuzhen"groups, wind the matching acupoint group and the original hole group big mouse large intestine originally organize SP-A mRNA expression and the model group compare, has the significance difference (p<0.05).(5) In the small intestine organization SP-A mRNA expression level does not have the significance difference between each group (p>0.05).(6) There are no SP-A mRNA expression has examined in the heart and in the stomach organization.Conclusion:(1) The acupuncture exceptionally breathes the function to the asthma big mouse to have the remarkable improvement function.(2) Asthma big mouse SP-A mRNA occurs exceptionally expresses and the breath function exceptionally has the relevance.(3) SP-A mRNA in big mouse lung and large intestine organization's expression existence relevance. The acupuncture participates in the adjustment asthma big mouse lung and the large intestine SP-A mRNA expression.(4) Winds the matching acupoint acupuncture method expression level adjustment to surpass the pure original hole acupuncture law originally to big mouse SP-A mRNA.