Through The Meridians Of The Particles Of The Preparation Process, Quality Standards And Preliminary Pharmacological Study

Having been offered clinically for many years, Tongjinghuoluo, a valid empirical prescription, has potent activity of huoxuetongluo and sanfengzhitong for treatment of Rheumatoid Arthrids, Numbness, and soreness, which in many occasions is satisfactory indeed.This article studied the extraction process, granulating process and quality standards of Tongjinghuoluo Granules. The Analgesic and anti-inflammatory activities of tongjinghuoluo were also dicussed.Extraction process study of Tongjinghuoluo Granules:A single factor experiment the concentration of alcohol extract of Achyranthes bidentata study goes on to the content of total saponin of Achyranthes bidentata indicators for the evaluation of the use of L9(3)4 orthogonal experimental design of Achyranthes alcohol extraction process of optimization, select the right Achyranthes bidentata extract greater impact on the number of extraction, extraction time, increase the amount of alcohol for research. As a results,3 numbers of extractions,1.5 hours every time,25 times amount of 80% ethanol, every time respectively.The use of L9(3)4 orthogonal experimental design to paeoniflorin, the total alkaloid content and extract yield index for the evaluation, select a greater impact on the water extraction process of the boiling number, boiling time, add water, soaked time to study. As a results,eight times amount of water, soaking 1.5h, decoction three times, each time the merger fried 1.5h liquid filtration.Processing teehnology study of Tongjing Huoluo Granules:The use of L9 (3)4 orthogonal experimental design study of the granulating process conditions, and the effects of humidity on the granulation factors. As a results, using 90% ethanol, the paste spray-drying from the extract was blended with crushed frankincense and myrrh,3:1 of the main drugs:dextrin,3% sucrose powder and 1% Steviosin. Then the blending was dried to get the tongjinghuoluo granules.Quality standards of Tongjinghuoluo Granulate:Quality standards of tongjinghuoluo was constited, content determined methods of paeoniflorin by HPLC.As a results,Identified methods of Radix paeoniae alba,Rhizom corydalis, Achyranthes bidentata blume, Phellodendron chinense, Puerariae radix, and Glycyrrhiza radix,Semen zizyphi spinosae was established by TLC.,method of determination of the content of paeoniflorin was established by HPLC.The study of the anti-inflammatory and analgesic activities of Tongjinghuoluo Granules:The anti-inflammatory effeet was tested by xylenc-induced ear oedma swelling in mice, foot-pad swelling caused by egg white in rats, The analgesie effeet was tested by paining by heating panel in mice, frequency body torsion caused by acetie acid in mice。As a result, to some extent the above models were all restrained by Tongjinghuoluo Granules, indieating Tongjinghuoluo Granules has anti—inflanunatory and analgesic effeetso...