Il-8 In The Tct Cervical Exfoliated Cells Expression And Significance

Careinoma of the uterine cervix that we are usually to see is the seeond commonest female cancer in the world, which only inferior to breast cancer. The prevalence lies in first plaee in our country and beeome one of the main disease which severely threaten health of women in our country. CIN is a cervical precancerous lesions,which is also an important stage of cervical cancer prevention and treatment,most squamous cell carcinoma from precancerous lesion.IL-8 have an important role in inflammation and tumor,which is also playa an important role in the development of cervical cancer as a cytokine.The subject to be studied the expression in cervical lesions of different degree by immunohistochemical methods.Purpose:To evaluate the expression of IL-8 in different level of TCT cells by immunohistochemical methodes,and to evaluate the difference of IL-8 expression on cervical exfoliated cells and cervical tissues.Method:To analyse and summarize patients, who voluntary to checkup TCT test in the First Hospital Affiliated of Shanxi Medical University from october 2008 to december 2009. Among them,the TCT test abnormal (i.e.:according toTBS system the pathological change above ASCUS) examples, which makes colposcopy test and takes the biopsy to carry on the pathological diagnosis have 71 examples. The 71 examples were divided into ASCUS, LSIL, the HSIL three groups by cytology,at the same time extracted 10 examples in the TCT test normal groups at random. Using the immunohistochemical methodes to detect the expression and trends of IL-8,and compare the difference of IL-8 expression in cervical cells and cervical tissues.Content:1. We collected TCT specimen 81 examples,which divided into four groups:NILM(n= 10), ASCUS(n=29),LSIL(n=32),HSIL(n=10);immunohistochemical staining for the expression of IL-8 in each group,the positive rate were 0,31.03%,62.50% and 70.00%.Comparison of IL-8 expression in the group of NILM and ASCUS, LSILand HSIL between the positive rate differences were significant.Comparison of IL-8 expression have no significant of the positive rate in each group ofASCUS,LSIL and HSIL2. Histological diagnosed CINⅡand CINⅢpercentage of 33.33% in IL-8 positive expression in the ASCUS,which percentage of 80% in IL-8 positive in the LSIL,100% in IL-8 positive in the HSIL,that the IL-8 used in the detectiom of cervical cytology will help detected CINⅡand CINⅢ.3. we collected 71 examples cervical biopsy tissue paraffin block of the TCT result abnormals, which divided into threer groups:inflammation or CINI(n=31),CIN II(n=19),CINIII(n=21); immunohistochemical staining for the expression of IL-8 in each group,the positive rate were 32.26%,78.95% and 85.71%.Comparison of IL-8 expression in cervicitis orCIN I,CIN II and CINIIIbetween the positive rate differences were significant.Comparison of IL-8 expression have no significant of the positive rate in each group of CINⅡand CINⅢ.4. Comparison of IL-8 expressed in CINII and CINIII have no significant in LSIL and HSIL of the cell block,comparison of IL-8 expressed in cervicitis or CINI have no significant in ASCUS of the cell block,showed that IL-8 expression in the cell block can reflect IL-8 expression in tissue.Conclusion:1.TCT cells prepared wax block can be used for screening of cervical lesions.2.IL-8 is not expressed in the cell of normal cervix, which is higher expressed in the cell of HSIL,so IL-8 use in cervical cytology to help the detection HSIL.3.The expression of IL-8 in cells of wax block can reflect in the expression of IL-8 in tissue,the positive expression of IL-8 in LSIL and HSIL suggest which histological diagnosis of CINII and CINIII,the positive expression of IL-8 in ASCUS suggest which histological diagnosis of cervicitis or CINI,but does not exclude CINII and CINIII.