| "Relation among the herbs, formula, effects and syndrome" is an important question in determination of treatment based in pathogenesis obtained through differentiation of symptoms and signs and Formulaology in TCM..The critical question in formulaology is effective principle,which involves in the herb dosage, compatibility,dosage form,sume methods and syndrome.When we make up formula,we must consider the effective object,dosage and compatibility,in this way the herbs can thoroughly educe the clinical effects.In this topic,the "Relation among the herbs, formula, effects and syndrome" is the question background,the effect of Bupleurum root on TOUXIETUIRE is the incision point. To research the effects of different dosages Bupleurum root on REZHENG model rars, we will search the dose-effect relationship of Bupleurum root on TOUXIETUIRE. Based on this research,we will compare with the Bupleurum root-baical skullcap root, Bupleurum root-chuanxiong and Bupleurum root-astragali radix on TOUXIETUIRE effects.Last, we aslo explore the effects of Bupleurum root on rats with diffent funcuion states.This topic is very important to probe the compatibility on dosage and herb selection,it is also significant to argument the clinical experience of Bupleurum root and reveal the modern connotation of TCM.Research 1:To make REZHENG model in ratsMethods:the male SD rat,220-250g,24; Male Kunming mouse,23-25g,20.We raise them for 3-5 days under the room temperature(25.C). In the 4th and the 5th days we will measure every day body temperature (anus warm) two times, then pick out the continual 2d single body temperature mouse surpasses 38.C, the rat surpass 39.C or two body temperature fluctuation surpasses 0.5. C which we does not need.Taking the mean value of the two body temperature as the foundational temperature. The male rats were assigned into normal group, the model group, the CHAIHU group,8 rats in every group. The male mouse were assigned into normal group, the model group,10 mouse in every group.To inject lipopolysaccharide (LPS)/NS solution (80μg/kg) intraperitoneally to induce fever in rats and mouse. At the same time, gives the bupleurum chinensis for the medicine group rat Shui Jianye 7.29g/kg (according to body surface area computation, namely according to person amount used 9g crude drugs'9 time of quantities fills the stomach every day), the normal group and the model group gives the same volume distilled water. The rectal temperature measured at Omin,60min,120min,210min,270min,330min after LPS injection in rats and Omin,30 min,60min,90min,120min,180min,240min, 330 min after LPS injection in mouse. Calculate the change rates of rectal temperature separately.Result:Compared with the normal group, the rectal temperature of the model group rat rises significantly(P<0.05或P<0.01); Compared with the model group, the rectal temperature of the CHAIHU group rats obvious drop, and at 60min,120min,210min,270min, the 330min the difference has the more significance (P<0.01). Compares with the normal group, the rectal temperature of the model group mouse elevates obviously, in time 60min,180min, the 330min the difference has remarkable or the significance significance(P<0.05或P<0.01), when 20min and 240min has raised tendency (the P value respectively is 0.077 and 0.068). Conclusion:Abdominal cavity injection 80μg/kg LPS may cause the rat and mouse's rise of bodily temperature obviously, and it gives off heat the duration to be long, may use in the observation that the medicine function bring down a fever; Experiment dosage's bupleurum chinensis Shui Tiye can give off the REZHENG rat model's heat. which causes to abdominal cavity injection 80μg/kg dosage's LPS and its temperature decrease function's duration is long.Research 2:Bupleurum Comparison of antipyretic effect of different dosesMethods:The body weight of male mice to 220-250g 5 days feeding adaptation, in the first 6,7 days of daily measured body temperature (rectal temperature) two consecutive 2d single temperature is above 38. C temperature fluctuations or twice more than 0.5. C, animals not removed, the average body temperature taken twice as basal body temperature, were randomly divided into normal, heat syndrome group, Bupleurum 0.27,0.81,3.24,4.86,7.29,9.72 g crude drug/kg dose group 6 (calculated according to body surface area, respectively, were 9g crude drug dosage/day 1/3,1,4,6,9,12-fold dose),12 in each group.)6h fasting before the experiment can not help but water. Model group and treatment group rats were injected with LPS (80ug/kg), induced animal heat; control group received normal saline. While modeling the Chinese medicine group were given the appropriate dose administered, the normal group and model group were given the same volume of distilled water, 1ml/100g weight. After drug administration for 0min,60min,120min,210min,330min rectal temperature measurement. The average temperature levels in each group.Result:Normal animals 30 minutes after modeling, a transient increase in body temperature occurs, then the level was reduced to normal levels; After modeling,60-330min, model animals body temperature was significantly higher compared with the normal group was significant difference (p<0.05), and the heat in 60min significant peak;) Treatment group animals, Bupleurum two low dose group and animals in two doses after the administration 60min,120min, 180min some lower temperature, but the overall difference was not significant; Compared with model group, one dose of Bupleurum animals body temperature and body temperature decreased after the modeling of 30-30min 120 minutes although there are lower but the difference was not significant,120-330min body temperature decreased significantly compared with model group, with significant difference (p<0.05); Bupleurum since freshman dose modeling, the temperature did not rise markedly, and the model group with significant difference (p<0.05), and continued to 330min; Bupleurum sophomore dose group after modeling, since, compared with the normal group significantly increased body temperature,30min peak,60min body temperature rather with the model group from 60min after the temperature decreased until the normal level,180-240min, and the model group with significant difference (p<0.05),330min, the with the normal group, the temperature was increased slightly.Conclusion:The bupleurum chinensis passes brings down a fever the function evilly with its amount used related, it brings down a fever at the effectiveness best dosage namely to be equal to person therapeutic dose 9 times, this uses in thoroughly brings down a fever when evilly the amount used experience basic match case with the clinical bupleurum chinensis.Research 3:Bupleurum Combination of different drugs on the antipyretic effect betweenMethods:Take consistent with the experimental conditions of male mice were randomly divided into normal group, model group, fever, Radix Group, Bupleurum-Scutellaria group, Bupleurum-Chuanxiong group and Chai Hu-RA group,10 in each group.6h fasting before the experiment can not help but water. Model group and treatment group rats were injected with LPS (80ug/kg), induced animal heat; control group received normal saline. While modeling the oral administration of Chinese medicine group, the normal group and model group were given the same volume of distilled water, 1ml/100g weight. After drug administration for 0min,60min, 120min,210min,330min measured rectal temperature, mean temperature levels in each group.Result:Normal animals in 30-60 minutes after the modeling had transient elevated temperature, then the level was decreased to normal levels; After modeling,30-330min, model animals body temperature was significantly higher compared with the normal group was significant difference (p<0.05), and the heat in 60min significant peak; Treatment group animals, Chaiqin animals there in 30 minutes when a transient temperature rise, then steady decline, down to normal levels 60 minutes, from 60 to 330 minutes, the basic little changed, with the model group with significant difference (p<0.01); Cai Qi group cooling effect is not obvious, from the line graph, but even higher temperature than the model group, but no significant difference, Cai Xiong group spite of the cooling effect, But not with the model group were significant.Conclusion:The firewood-qin medicine right LPS suggestion's mouse gives off heat has obvious brings down a fever the function, the firewood-qi and the firewood-xiong medicine right this model obvious has not brought down a fever the function, the bupleurum chinensis passes brings down a fever the function to blend closely evilly with it related.Research 4:Bupleurum best compatibility on different state (model) temperature of miceMethods:Take consistent with the experimental conditions of male mice were randomly divided into the normal group, the model group, the normal bupleurum chinensis group, normal Chai Qinzu, the model bupleurum chinensis group, model Chai Qinzu stochastically, each group of 12.6h fasting before the experiment can not help but water. While modeling the oral administration of Chinese medicine group (subject to specific doses and combined with previous experiments to be), the normal group and model group were given the same volume of distilled water, 1ml/100g weight. After drug administration for Omin,30min,60min,120min,180min, 240min,330min measured rectal temperature, mean body temperature levels in each group.Result:(1) the normal group animal after filling the stomach when 30min the anus appears warm one has elevated, the 30-60min body temperature drops gently, the 60-120min anus presents a platform time warm, the 120-330min anus warm gentle drop to the normal level; For the medicine group animal, after filling the stomach, with normal group compared to, normal Chai Qinzu mouse anus warm obvious drop, when 30min presents the first low temperature peak value,30-120min, the anus has warm elevates, but was still lower than the normal group animal anus to be warm, then slowly drops, when to 180min, presents the second low temperature peak value, when to 240min, the anus warm change value and the normal group quite,240-330min, the anus warm again reduces, presents the third low temperature peak value; Normal Chai Qinzu the mouse anus is warm,0-240min reduces obviously,240-330 anuses appear warm elevate the tendency, and the anus warm change value is higher than the normal group mouse.2) The normal group animal after filling the stomach when 30min the anus appears warm one has elevated, then gradually drops to the normal level. Compares with the normal group, the model group animal anus heats in 30-240min obviously elevates, and appears obviously in 60min gives off heat the peak value. Compared with the model group, the model bupleurum chinensis group big mouse anus warm drop is obvious, and presents two obvious low temperature peak values with 60 and 330min; Model Chai Qinzu big mouse anus warm drop, but its change value curve occupies the model bupleurum chinensis group throughout the place above, is inferior to the model bupleurum chinensis group to be obvious.Conclusion:The bupleurum chinensis and bupleurum chinensis-Huang Qin blends to the normal big mouse and the hot card model big mouse has the varying degree temperature decrease or brings down a fever the function, but the temperature decrease function has the characteristic respectively. The result prompt, the bupleurum chinensis and bupleurum chinensis-Huang Qin blends passes brings down a fever effectiveness to concern evilly with its function object condition.Research 5:The bupleurum chinensis and bupleurum chinensis-Huang Qin blends brings down a fever the mechanism the part investigationMethods:Takes conforms to the condition SD rat, divides into the normal group, the model group, the bupleurum chinensis group, Chai Qinzu stochastically, each group of 10. Before the experiment,6h fasts to be unable to restrain the water. The abdominal cavity injects LPS at the same time bupleurum chinensis group dosage to fill the stomach according to 7.29g the crude drugs/kg, Chai Qinzu dosage fills the stomach according to 12.76g the crude drugs/kg, the normal group and the model group gives the same volume distilled water to fill the stomach. After Guanwei Omin,60min,120min,180min determine each group of big mouse's anus to be warm. Each group of big mouse After Guanwei when 210min beheads draws blood 2ml, in 4. Under C the 3000r/min offcenter, is accepted after passing an examination the level blood serum, puts in-80. C refrigerator; And regards the overlapping center point according to the brain atlas take the tuber cinereum as the limits spot, takes the big mouse hypothalamus organization to set in rapidly the liquid nitrogen quick-freeze fixed, after 20min, puts in-80. C refrigerator, testing.Result:(1) Compared with the normal group, after the model group animal abdominal cavity injects LPS, the 60-180min period's anus warm assumes elevates, and in time 60min and 180min appears obviously gives off heat the peak value; Compared with in the model group, after Chai Qinzu and the bupleurum chinensis group animal abdominal cavity injects LPS, the 60-180min period anus warm obviously reduces; Compared with Yu Danwei bupleurum chinensis group, Chai Qinzu animal after the abdominal cavity injects LPS 60min the anus temperature drop low is more obvious. (2) Compared with the normal group, in the model group big mouse circumference blood the IL-6 content not obvious difference, the TNF content obvious drop, the difference has the significance significance (P<0.05); The hypothalamic PGE2 content assumes elevates the tendency (P=0.083), AVP and cAMP have not seen the significance change (P>0.05). Compared with the model group, the bupleurum chinensis group and Chai Qinzu in the big mouse circumference blood the IL-6 content not obvious change (P>0.05), the TNF content obvious drop, the difference has the significance significance (P<0.05); Two group of big mouse hypothalamus cAMP has not seen the obvious change, the PGE2 content has reduces the tendency (P>0.05), the AVP content has the varying degree to elevate, the difference has the unusual significance significance (P<0.01).Conclusion:The LPS induction's rat gives off heat mainly involves the main center to adjust the mechanism, involves the main center many kinds of sends the hot quenching and tempering (positive and negative quenching and tempering) combined action. The bupleurum chinensis brings down a fever the function possibly mainly with to reduce in the hypothalamus PGE2 related, firewood-qin medicine to brings down a fever the function possible with to reduce in the brain the PGE2 content and elevates in the brain the AVP content related, prompts both to bring down a fever in the function mechanism to differ from.This topic from the experiment angle, inspected the bupleurum chinensis dosage, to blend with the adaptation card brings down a fever the function to it the influence, discovered that the bupleurum chinensis brought down a fever the function with its amount used, to blend and the function object concerns. This research to prove between the Chinese medicine-side-card-effect the inner link to provide certain modern basis, has provided certain evidence for on the understanding Chinese medicine clinical bupleurum chinensis using experience's rationality. |
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