| The Minor Chaihu Decoction is the representative formula for harmonizing Shaoyang, which is usually used to treat the diseases of digestive system, nervous system, respiratory system, etc. The relative modern investigations have indicated that this formula has many effects, such as anti-febrile, anti-inflammation, hepatoprotection. The paired Chinese herbs, the Bupleurium root and Scutellaria root, act as the core conformation of the Minor Chaihu Decoction, possessing the important effect of "dispersing the evil in exterior and clearing away the heat in interior" and embody the main functions of the Minor Chaihu Decoction. The recent researches have proved that the Bupleurium root, Scutellaria root and the components in both herbs also have the effects of anti-febrile, anti-inflammation, hepatoprotection, which suggest that the paired Chinese herbs of Bupleurium root, Scutellaria root is the most important part contributing to the pharmacological functions of the formula. In this experiment we identified the difference in pharmacological effects of the decoctions of paired Chinese herbs, Bupleurium root and Scutellaria root, decocted separately and mingledly respectively, and separated the main chemical component clusters within the decoction which has stronger effects, then tried to find out the relativity for material foundation and the protection on the experimental febrile and acute liver injury. This experiment is mainly divided into three parts. 1 The difference in pharmacological effects between the water extractions of Bupleurium root and Scutellaria root (4:3) decocted mingledly and separately, respectively.Objective: To compare the activity of both decoctions on experimental febrile and liver injury. Methods: (1) To inject lipopolysaccharide (LPS) /NS solution (20μg/kg) intraperitoneally to induce fever in rats, and administrate the both decoctions orally at dose of 10g/kg immediately after the LPS injection. (2) To inject LPS/NS solution(80μg/kg) intraperitoneally and orally administrates the both decoctions at dose of 10g/kg, 120min after LPS injection. The rectal temperature was measured every 30min after LPS injection, observing 330-450min continuously. Calculate the change rates of rectal temperature (ΔT). CCl4 intraperitoneal injection was used to induce the rat acute liver injury. Before duplicating the model, the rats in treat group were given the mingled decoction and separate decoction orally at dose of 10g/kg, once a day and continuing 5 days. 2hrs later since the last administration, 50% CCl4/vegetable oil was injected intraperitoneally at dose of 2ml/kg to induce the acute liver injury in rats. Collect the blood 24hr later, and measure the activity of ALT, AST, ALP, and the concenttation of TBIL in serum. Results: The results indicate that LPS can significantly elicit the rising of the rectal temperature. Its ΔT-time curve appears two phases when injected with LPS at does of 20μg/kg and appears three phases when LPS at dose of 80μg/kg, and the last peak is highest. Bupleurium root and Scutellaria root mingled decoction and separate decoction can significantly inhibited the fever induced by LPS at both dose (P<0.05 or 0.01), and there is no significance between the both decoctions. But analyzing the effects of anti-febrile of both decoctions synthetically, we found that the mingled decoction presented the anti-febrile effect earlier than the separate decoction, and indicated the better efficacy in trend. In the hepatoprotection research, we found that both of the decoctions can significantly inhibit the increase of ALT and AST in serum (P<0.001), indicating that the both of decoctions have the certain effect of hepatoprotection. In addition, the mingled decoction also can decrease the activity of ALP and the concentration of TBIL (P<0.05), while the separate decoction has slight effects on ALP (P>0.05). It suggests that these two decoctions influence on different link of the effect of hepatoprotection, and the mingled decoction be better than the separate decoc... |
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