Stagnation And Spleen Deficiency Syndrome Model Rats, The Mtl, Gas, Stomach Rationale Morphological Changes And Xiaoyaosan Of Regulation,

1 Thoretical StudyStress is a serial physiological-biochemical reactions which emerge to enhance the organism adaptivity and to mentain the stability of inner-environment when the organism is intensively stimulated. chronic stress will cause somatopsychic disturbance in various degree, and cause desease. Oragnism under chronic stress would cause change in many aspects in internal human organism,physiology,biochemistry, and would involve multipe system. Traditional Chinese Medicine treatment method shows her advantage and characteristic for prevention and curestress disease. This topic simulate the pathology devloping process of a disorder due to emotional upsets or other factors for a long time, Woods Restricts Earth, a morbid condition of hypofunction of the spleen and the stomach resulting based on model of stessed rats in chronic restrict method. Xiaoyaosan is the classic prescription for curing Liver invading the spleen syndrome, this experiment is start from Xiaoyaosan, investigate the control response of Xiaoyaosan.Gastrin is secreted by G cells of sinuses vntriculie, and has intensive stimulation to cell in stomach wall which secret gastric acid, and increase the secretion of other matter and the nutritional protection of gastrointestinal tract die sticking. Deficiency of the Spleen,Stagnation of the Liver-Qi,Liver invading the spleen syndrome all could cause the declination of blood serum gastrin level, however, by effective treatment, the consistency in blood serum gastrin can be thickened by effective treatment, thus improve the symptom. Motillin is a kind of braingut petide, which is secreted by EC cells in small intestine, which spread in gastrointestinal tract die sticking and nervous system. Human and mamals limosis gastrointestinal motility mode is MMC, which of the mechanism is launched by motillin. Existing documents have reported that Stagnation of the Liver-Qi functional indigestion model, Liver invading the spleen syndrome model and chronic stress rats have distinctively lower plasma motillin level than normal mode have, as well as reported that Deficiency of the Spleen-Yin model rats have upper plasma motillin level than normal mode.2 Experimental StudyThis experiment is consist of two parts. The first experiment revealed that mode through chroni restrict stress are mode of Liver invading the spleen syndrome of traditional chinese medicine by animal macroscopy token, diet and weight change, objective indicator contents of serum and D-xylose and intervening effect of Xiaoyaosan; The second experiment researched the change of GAS,MTL in mucous membrane of rats'sinuses ventriculi that under twenty-one days' choronic restrict stress, and pathology change in stomach organism, for the purpose of discussing the change of gastrointestinal hormone in Liver invading the spleen syndrome mode rats and the control response of Xiaoyaosan. Rats was splitted into three groups:normal, mode and Xiaoyaosan group. Each has eighteen rats, and three per cage. binding 21d, Except the Xiaoyaosan group which is drenched with Xiaoyaosan, the normal and mode group are drenched with the same volumn of physiological saline. After modeling, the radioimmunity analytical method is used for testing rats'peripheral blood and consistency of motillin and gastrin in intestines and stomach mucous membrane; observe the morphological change in rats'stomach mucous membrane.3 Result and Conclusion3.1 Experiment 1Result:mode group rats changes from strongly resisting when first bond to tired and listless with furs scroch, dispersive and easy to fall, with secretion in eyes, fond of flocking together, and unresisting; mode gorup rats dislike diet, and have slow body weights growth as well as declination of consistency of plasma D-xylose, however, Xiaoyaosan could improved these related figures in mode group rats, and have distinctively control response.Conclusion:the stress rats model which using chonrinc restrict method to simulate the disorder of emotional upsets or other factors, cause some morbid condition of hypofunction of the spleen and stomach resulting, of cause of disease of traditional chinese medicine, has successfully reproduced the Liver invading the spleen syndrome rats mode.3.2 Experiment 2Result:After binding the rats 21 days, the model group rats'density of the GAS and MTL in blood and gastric antrum can decrease, while Xiaoyaosan group's is similar to the normal group's. Xiaoyaosan plays a very good regulation. we can see edema,congestive and chronic inflammation in the gastric mucosa about the model group rats. Xiaoyaosan group have only minor pathological changes, epithelial damage and defect-free, non-inflammatory exudate, and the muscle fibers are arranged regularlly.Conclusion:model group rats'GAS and MTL in blood and tissues can decrease. The gastric mucosa can have a certain degree of edema, congestion, and chronic inflammatory pathology. Xiaoyaosan can effectively fight against such changes. Xiaoyaosan plays a good regulation to Liver invading the spleen syndrome.