Yangyinqingfei Law Treatment Of Lung Cancer Ppar¦Ã Signaling Pathway

The incidence of lung cancer in China has continued to rise, in Beijing, Shanghai and other big cities, the incidence and mortality of lung cancer account for a variety of malignant tumors of the first. At present the treatment of advanced lung cancer radiotherapy and chemotherapy are still the main, albeit short-term effect, but only for patients in better physical condition. Significant side effects associated with chemotherapy, and remission is short, the immune f unct ion of patients and disease resistance often bring injury, serious impact on quality of life. Chinese medicine treatment side effects, efficacy and stability, can improve the quality of life for advanced lung cancer, especially Yangyinqingfei method has been recognized by the majority of clinical experts.PPARγisⅡtype nuclear hormone receptor superfamily member. A lot of literature reported that PPARγwere expressed in many tumor tissues,such as lung cancer, colon cancer, breast cancer, gastric cancer, pancreatic cancer, Activated by the ligand it can play a role in anti-tumor and it is expected to be a new cancer treatment drug target. To study signal transduction pathways may provide new ideas for cancer treatment.Objective: Clinical study found that Pingfei which is created by LiPeiwen professor under the rule of law Yangyinqingfei is one of the Independent prognostic factors to extend survival in patients with lung cancer, but it lack of in-depth study. This study explored the treatment of lung cancer Pingfei mechanism to identify the possible drug target.Method:Use the Lewis lung cancer model of tumor-bearing mice.24 C57 mice were randomly divided into four groups:normal control group, model group and cisplatin group were administered in the first day after modeling with normal saline 0.4ml×15 days; cisplatin+Pingfei group was administered in the 1st day after modeling with Pingfei Mixture 0.4ml (equivalent amount of crude drug 28g/kg)×15 days; both of cisplatin group and cisplatin+Pingfei groups were injected cisplatin injectionused 1ml (including DDP0.1mg) intraperitoneal ly in the 7th,9th,11th day. After the second day administered, concentrat serum, Strip tumor, and weighed to calculate tumor inhibitory rate. Using Double antibody sandwich ABC-ELISA method to detect adiponectin. Using immunohistochemical staining to detect PPAR-γ/ P27/VEGF and ki-67 protein expression.Results: Inhibition rate:cisplatin group and cisplatin + Pingfei group's tumor weight significantly less than model group (P<0.01), while cisplatin and cisplatin + Pingfei's tumor weight was no significant difference (P> 0.05), but cisplatin + Pingfei group had decreased weight trend. Compared to cisplatin group. Serum adiponectin level:Compared with normal group, the tumor-bearing mice group, the average levels of adiponectin were significantly decreased (P<0.01). Among the tumor-bearing mice group, the level of adiponectin of the cisplatin group was slightly higher than the level of adiponectin model group, but no significant difference (P> 0.05), while the level of adiponectin of cisplatin + Pingfei group was significantly higher than model group and the cisplatin group (P<0.01). PPAR-γprotein expression: Cisplatin compared with model group, PPAR-γexpress more (P<0.05). Cisplatin + Pingfei group compared with model group, DDP group, PPAR-γexpression increased significantly (P<0.01). P27 protein expression: cisplatin group compared with model group, P27 protein expression increased slightly, but not statistically significant (P> 0.05). Cisplatin + Pingfei group compared with model group and cisplatin group, P27 protein expression increased significantly, the differences were statistically significant (P <0.01). VEGF protein: VEGF expression in model group was significantly higher than cisplatin and cisplatin + Pingfei group (P<0.01), while the expression of VEGF of cisplatin + 1 Pingfei group was significantly reduced compared with cisplatin (P<0.01). Ki-67 protein expression: the ki-67 expression of model group was significantly higher than cisplatin and cisplatin + Pingfei group (P<0.01), while the expression of Ki-67 of cisplatin + Pingfei group compared with cisplatin there was nothing no significant difference (P> 0.05), but the trend is becoming smaller.Conclusion: Pingfei can raise the expression of PPARγ, P27, adiponectin and the inhibition of VEGF expression to play a role in treatment of cancer, PPARγis one of the drug target of Yangyinqingfei.