Qudu Tang Induced In Patients With Anll-cr The Bmnc Source Dc

Objective:The study intends to use the method of in vitro,he study intends to use the method of in vitro,culture the TD-BMNC of ANLL-CR Patients of complete remission standards with the Medicated Serum of Qu Du Tang and Cytokines,observe the effect on the differentiation and maturation of monocyte-derived dendritic cells by the Medicated Serum. culture the kind of DC and the purified Perpheral blood T-cellof the Normals and ANLL-CR Patients,observe the effect on the proliferative capacityof the T-cell. culture the excited T-cell and Leukemia Cell Lines K562 together, measure the cytotoxic activity of T-cell on Leukemia Cell Lines K562 by the MTT method, study the effect of Qu Du Herbs on the immune function of patients DC with acute myeloid leukemia .Methods: First, the TD-BMNC by the Method of Sheep Red Blood Cells Separating ANLL-CR Patients T Lymphocytes, Prepare the Medicated Serum of Qu Du tang, the TD-BMNC were cultured in RPMI1640 containing 10% fetal bovine serum (FBS)in six- orifice plate with different concentrations of the Medicated Serum and cytokines(GM-CSF,IL-4,TNF-α)。Second,the shape of cell was observed during cell cultured. Third,Flow cytometry was used to analyze surface marker of CD1a CD86and HLA-DRof DC.Fourth, The different kinds of T-cell from the Normals and ANLL-CR Patients and this kind of DC from the TD-BMNC were collected and inoculated in 24- orifice plate,with the Proportion of 10:1。exciting the two different kinds of T-cell with IL-2(100U/ml)in RPMI1640 containing 10% FCS。Fifth ,the differet excited T-cell and Leukemia Cell Lines K562 are cultured with the Proportion of 10:1;20:1; 40:1,measure the cytotoxic activity of differnt excited T-cell on Leukemia Cell Lines K562 by the MTT method.Results:1 The shape of cell was no difference each group of between DCwith psoriasis vulgaris.2 Qu Du soup various monitoring teams, the cell factor union QuDu tang various monitoring teams, the cell factor system and the blank blood serum group induces DC CD-1a, CD86, the HLA-DR expression comparison has the remarkable difference (P<0.01), surpasses the blank blood serum group; Qu Du Tang small dosage, Qu Du soup the monitoring team and the cell factor system induce DC the CD-1a expression to have the difference (P<0.05),Qu Du Tang, cell factor union Qu Du Tang small dosage, the monitoring team and the cell factor system CD-1a expression has the remarkable difference (P<0.01); The cell factor union QuDu Tang big monitoring team has the difference with Qu Du Tang big monitoring team CD-1a (P<0.05); The cell factor union Qu Du Tang big monitoring team and cell factor union Qu Du Tang small dosage, the monitoring team induces DC CD-1a to have the difference (P<0.05).Qu Du Tang big monitoring team and the cell factor system induces DC the CD86 expression difference (P<0.05); QuDu Tang big monitoring team induces DC CD86 with QuDu Tang small monitoring team the expression to have the difference (P<0.05) QuDu Tang small monitoring team, the QuDu big monitoring team and cell factor union Qu Du Tang small monitoring team induces DC HLA-DR the expression and the cell factor system induces DC HLA-DR the expression to have the remarkable difference (P<0.01), The cell factor union Qu Du Tang big monitoring team has the remarkable difference with Qu Du Tang big monitoring team (P<0.01).3 The different density group separately do not have the remarkable difference with the blank blood serum group kill rate (P<0.01), surpasses the blank blood serum group; QuDuTang union cell factor small, medium and big monitoring team, Qu DuTang big, middle and QuDuTang small monitoring team has difference P<0.01, surpasses QuDuTang small monitoring team. In group comparison: in the normal person T cell ,Qu Du Tang monitoring team 40:1 and 10:1 effect target has the remarkable difference compared to the kill rate (P<0.01), Other groups does not have the difference, Qu Du Tang monitoring team 20:1 and 10:1 effect target ratio has the difference (P<0.05); Qu Du Tang the dosage union cell factor system 10:1 and 40:1 effect target compared to has the difference (P<0.05), Qu Du Tang the dosage union cell factor system 20:1 and 40:1 effect target ratio has the difference (P<0.05), others the effect target ratio does not have the difference.In the patient T cell :Qu Du Tang small monitoring team effect target compares 40:1 and 10:1 has the difference (P<0.05), Others the effect target compares does not have the difference (P>0.05).Conclusion:1 marrow single nuclear cell the blood serum induction raise may obtain after Qu Du Tang has the typical characteristic and cell phenotype DC.2 Qu Du tang induction DC activates the T lymphocyte to be able the special killing K562 tumor cell.