| Object: significance acute leukemia is the threaten seriously patient life malignant nosohemia, along with the modern chemotherapy method's development, the new chemotherapy medicine's development as well as the hematopoietic stem cell transplant's development, its alleviation rate had the very big enhancement, some cases have cured, but many patients still unavoidably recurred endanger life. After the leukemia recrudescence's basic reason is the alleviation, patient in vivo still had the micro leukemia cell, but organism cannot carry on the immunity surveillance to the residual leukemia cell to eliminate it. Enhances the patient life even to cure the leukemia, the remission treatment appears especially of important, residual leukemia cell's elimination into key, this is also the present research one of key point and hot spots. However, still lacked the alleviation, after at present leukemia namely small residual leukemia (Minimal Residu-al Disease in Leukemia, MRD-L) the effective method of treatment, discusses its method of treatment from the Chinese medicine angle to have the important meaning without doubt.method :with adds taste southernwood carapax trionycis Tang Guanwei the animal (big white rabbit) to prepare the traditional Chinese medicine to contain the medicine blood serum spare. Will achieve alleviates the standard completely the acute leukemia patient marrow after the antifreeze, the application sheep red blood cell rose chou procedure from the marrow separates T cell's marrow single nuclear cell (TD-BMNC), adds taste southernwood carapax trionycis Tang Hanyao the blood serum and the cell factor union raise, observes including the medicine blood serum the tree suddenly shape cell differentiation mature situation influence which originates to ANLL-CR patient TD-BMNC. With the normal person who or the remission patient circumference blood T cell purifies raises together with this law induction differentiation's tree suddenly shape cell, observes to the T cell multiplication ability influence. Cultivate together with the stimulation T cell and the K562 cell, determines this T cell by the MTT law to the K562 cell's killing activeness.Result: 1.DC raise and under observation inverted microscope: Trains 4d obvious cell Cheng Cu to grow, becomes the anomalous shape by the circular. Raises 7d, grows some small spinel structures, the cell qualitative change richly, and grows the tree suddenly type pseudopod, raises the 9th day of tree type structure to be suddenly more obvious, the cytoplasm is richer.2. In these three kind of raise system, the ANLL-CR patient goes to T cell's marrow MNC (TD-BMNC) to be able to split up into shape characteristic model DC, can express mature DC the specificity mark (CD-86 and CD-1a), expresses HLA-DR high. Adds the taste southernwood carapax trionycis soup to gather the cell factor system the CD-1a expression to be higher than the cell factor system and to have the remarkable significance; The large dose Canadian taste southernwood carapax trionycis soup gathers the cell factor system the CD-86 expression to be higher than the cell factor system and to have the remarkable significance; The large dose Canadian taste southernwood carapax trionycis Tang Zu CD-86 expression is higher than the cell factor system and has the remarkable significance; The large dose Canadian taste southernwood carapax trionycis soup gathers the cell factor system the HLA-DR expression to be higher than the large dose the Canadian taste southernwood carapax trionycis soup group; The large dose Canadian taste southernwood carapax trionycis soup gathers the cell factor system the HLA-DR expression to be higher than the cell factor system.3. In the effect target compared to 10:1, the large dose adds the taste southernwood carapax trionycis soup to gather the cell factor system (normal person T cell group) the target cell's kill rate with to add taste southernwood carapax trionycis Tang Zu (normal person T cell group) to compare has the remarkable difference; The large dose adds the taste southernwood carapax trionycis soup to gather the cell factor system (normal person T cell group) the target cell's kill rate and the cell factor system (normal person T cell group) compares has the remarkable difference; The large dose Canadian taste southernwood carapax trionycis soup gathers the cell factor system (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference; Large dose Canadian taste southernwood carapax trionycis Tang Zu (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference; In the effect target compares in 20:1, the dosage Canadian taste southernwood carapax trionycis soup gathers the cell factor system (patient T The cell group) the target cell's kill rate (patient T cell group) compares with dosage's Canadian taste southernwood carapax trionycis Tang Zu has the remarkable difference; The dosage Canadian taste southernwood carapax trionycis soup gathers the cell factor system (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference; The large dose Canadian taste southernwood carapax trionycis soup gathers the cell factor system (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference; The large dose Canadian taste southernwood carapax trionycis soup (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference; In the effect target compared to 40:1, the dosage adds the taste southernwood carapax trionycis soup to gather the cell factor system (patient T cell group) the target cell's kill rate and the cell factor system (patient T cell group) compares has the remarkable difference.conclusion: adds the taste southernwood carapax trionycis soup to gather the cell factor altogether to raise can urge DC to be mature, and compares with the pure cell factor raise, has the more remarkable dialog hemopathy cell's kill rate. We may through add the taste southernwood carapax trionycis soup union cell factor to induce DC to split up maturely enhance the dialog hemopathy cell's specificity killing activeness, alleviates after the acute leukemia sought the effective method of treatment, waits for further studying in the clinical practice. |
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