Pharmacodynamic Study Of Diabetes Pill Mechanism Of Its Hypoglycemic Effect

Purpose: To observe the hypoglycemic effect of Tangniaokang pills, come tounderstand the acute toxicity of Tangniaokang pills preliminarily, and explore the hypoglycemic mechanism that Tangniaokang pills effect.Materials and methods : To observe the effect of blood sugar whichTangniaokang pills make on the experimental diabetes model mice and glucose and adrenaline-induced hyperglycemia in mice that were caused by alloxan. Using the radioimmunoassay to evaluate the effect which Tangniaokang pills make on the content of the experimental diabetes model mice serum insulin that were caused by alloxan. With anthrone color mensuration to evaluate the effect which Tangniaokang pills make on the content of the experimental diabetic mice liver glycogen.Results:1. Acute toxicity test: The largest drug delivery content of Tangniaokang pill oral administration is 41. 93g pharmacognostic / kg, according to the clinical equivalent conversion kg dosage (17. 28g pharmacognostic / person / day = 0. 247g pharmacognostic / kg) of 170 times. Mice show no abnormal and no dead case.2. Pharmacodynamics experiment: Tangniaokang pills Middle dose group (9.0g pharmacognostic /kg) can reduce 7days blood sugar content of experimental diabetic mice which were caused by alloxan significantly, Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg ),Middle dose group ( 9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic/kg) can reduce 14days blood sugar content of experimental diabetic mice which were caused by alloxan significantly; Tangniaokang pills Low-dose group (4. 5g pharmacognostic /kg),Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg) can reduce the blood sugar content of hyperglycemia experimental diabetic mice; Middle dose group (9.0g pharmacognostic /kg) and High-dose group (18.0g pharmacognostic /kg) can reduce the blood sugar content of the adrenaline-induced hyperglycemia mice which were injected adrenaline 0. 5 hours significantly; Tangniaokang pills Low-dose group (4. 5g pharmacognostic /kg),Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg ) can reduce the blood sugar content of the adrenaline-induced hyperglycemia mice which were injected adrenaline 1 hours later significantly ; Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg),Middle dose group (9.0g pharmacognostic/kg) and High-dose group (18.0g pharmacognostic /kg ) can increase the serum insulin levels of the alloxan-induced experimental diabetic mice significantly; Tangniaokang pills Low-dose group (4.5g pharmacognostic /kg ),Middle dose group (9.0g pharmacognostic /kg)and High-dose group(18.0g pharmacognostic /kg)can increase the liver glycogen content of the alloxan-induced experimental diabetic mice significantly.Conclusions:1. Acute toxicity test: Tangniaokang pills are safe for oral drug delivery.2. Pharmacodynamics: Tangniaokang pills can significantly reduce the blood sugar levels of experimental alloxan-induced diabetic mice, can significantly reduce the blood sugar levels of the experimental mice which have reduction in blood sugar, improving the glucose tolerance of the normal mice; probably increase the serum insulin content of the alloxan-induced experimental diabetic mice by reducing alloxan-induced isletβcell damage, repairing damage of isletβcell; probably increase the liver glycogen content of the alloxan-induced experimental diabetic mice by inhibiting glycogenolysis, promoting the glycogen synthase.