Total Saponins Of Notoginseng Aerial Parts Of The Hydrolysis Products Of Pharmaceutical Research

In this paper 11 compounds were isolated from the hydrolysates of the stems and leaves of Panax notoginseng(Burk) F.H.Chen by modern technologies of extraction,isolation,purification and identification,such as AB-8 macroporous absorption resin (MAR) , silica gel-column chromatography,R-phase high performance liquid ( RP-HPLC-UV-ELSD) and re-crystal.the structures of 11 compounds have beenestablished and 10 compounds have identified on the basis of physico-chemical and spectrographic evidence in the hydrolysate of the stems and leaves of Panax notoginseng(Burk) F.H.Chen. They were dammarane-20(22)en-3β,12β,25-panaxatriol(Ⅰ),20(R) -dammarane-3β,6α,12β,20,25-pentol(Ⅱ),20(R)-dammarane-3β,12β,20,25-triol(Ⅲ),20(S)-panaxadiol(Ⅳ),20(S)-protopanaxadiol (Ⅴ),20(R)-protopanaxadiol (Ⅵ),20(S)-panaxatriol(Ⅶ),20(S)-25-methoxyl-dammarane-3β,12β,20-triol (Ⅷ),20(S) -25- ethoxyl-dammarane-3β, 12β, 20-triol(Ⅸ),20(R) -25- ethoxyl–dammarane-3β, 12β, 20-triol(Ⅹ) .Among them compoundⅨandⅩwere found as the new product.using high performance liquid chromatography(HPLC) with evaporative light scattering detection(ELSD) determinate the quantitative of 20(S)-PD,20(R)-PPD,20(R)-25-OH-PPD in different parts hydrolysates of Panax Notoginseng。The results show that 20(S)-PD,20(R)-PPD,20(R)-25-OH-PPD are all highest in the hydrolysate of the stems and leaves of Panax notoginseng(Burk) F.H.Chen.Study systematically on the anticancer activity of 20(R)-PPD ,20(R)-25-OH-PPD,The result of vitro screening of pharmaco-activity study showed that, 20(R)-PPD,20(R)-25-OH-PPD showed obvious inhibition activity on tumor cells by used five cell system including breast cancer cells,liver cancer cells,prostatic carcinoma cells,colon cancer cells and lung cancer cells.