Strychnos Alkaloids On The Morphological Ultrastructure Of Guillain-barre Rabbit Peripheral Nerve Pathology

Objective:To observe the influence of Maqianzi of total alkaloids for the Guillain-Barre syndrome classical animal model-acute allergy neuritis domestic rabbit's peripheral nervepathology morphology ultrastructure;to discuss the effect of the main effective ingredient in Maqianzi-Maqianzi of total alkaloids' curative function for for the GBS classical animal model;to make the poisonous tradional Chinese medicine-Maqianzi apply to clinical medicine better.Method:We intensively mixed the cow Myelin Basic Protein 16ml and theisometric Complete Freud's adjuvant,the immuriity domestic rabbit prepared the EAN animal model.Model Group for the pathogenesis of rabbit,with the first 21 days 8ml MBP,and contour incomplete Freund's adjuvant fully mixed peritoneal do additional immunization.This experiment totally used 32 domestic rabbits which divided into four groups.8 normal healthy domestic rabbitsare the blank group,the blank group replaced the cow MBP andisometric CFA by the 16ml physiological saline carry on theimmunity to take the comparison.Another modeling success from the EAN 24 rabbits were randomly divided into model group, Maqianzi of total alkaloids treatment group,hormone therapy group,n=8.Maqianzi of total alkaloids given oral treatment group Maqianzi of total alkaloids of 0.05mg/kg/day treatment group were given hormone ear vein injection of dexamethasone 0.5 mg/kg/day treatment.Respectively,after the onset seven days based on experimental animals,the application of electron microscopy peripheral nerve Pathomorphology ultrastructural changes.Result:Electron microscopy observation of the sciatic nerve rabbit model group for the major features of demyelination and myelin swelling.Mitochondrial swelling,cell swelling, Schwann cell nuclear pyknosis,cytoplasmic matrix cavitation.After treatment,the Maqianzi of total alkaloids of the treatment group and hormone treatment group myelin loss situation has markedly improved,clarity of myelin lamellar increased,the basic structure of large nerve fibers in normal Schwann cell reduce swelling,enlargement of individual mitochondria,cells restore basic form,and a new small myelinated nerve fibers generation.Conclusion:Oral-Maqianzi of total alkaloids-can significantly improve the EAN rabbit sciatic nerve myelin swelling and loss,and reduce mitochondrial swelling of Schwann cells, the basic restoration of cell morphology.