Gynostemma Cell Culture And Its Extracts Hypoglycemic Activity

Objective To Gynostemma callus cells were used to explore the cell growth regulation,e main types, and their hypoglycemic effect.Methods Gynostemma callus cells were cultured in MS medium having no plant hormones for 36 days. Weighed the cultured cells for fresh weight every three days, calculated growth index, and protracted cell proliferation growth curve. Harvested cells in the harvest period, extracted saponins from the cells adopting alcohol references, colorimetry was applied to measure the content of their saponins, and mass spectrograph detected saponin type. Cell extracts were used in 18 ~ 22g BALB / C mice in the method of intraperitoneal injection and oral gavage. Glucose detection kit was used to detected the hypoglycemic effect.Results The growth cycle of the gynostemma callus cells was 27 days, and was S-shaped curve, can be broadly divided into delay period, the index growing period and quiescent period. Appropriate vaccination weight is 1.2 g/40ml MS solid medium. The content of saponins of the harvest period cells was 1.02% (w/w) from the colorimetry. The glucose detection kit was used to detect the blood glucose of the BALB / C mice, there was a certain degree of hypoglycemic effect.Conclusion After studying the Gynostemma callus cell growth dynamics regulation, determined its growth curve and the best harvest period is 25~27th days and the best transition period is 21~24th days. Detected the saponins content and their type. Confirmed that Gynostemma Callus cells have certain hypoglycemic effect.