Sinomenine Intervention Tumor Cell Apoptosis, Erk-bcl-2 Signal Transduction Mechanism

Objective:To research SIN intervention the signal transduction of tumour cell.Study different dose SIN induce apoptosis of tumour cell. Methods:Hela cell were incubeded in vitro.This cell were divided into 3 experimental groups and 1 control group.The experimental group were given RPMI1640 with different dose SIN.The control group were given RPMI1640 without SIN.After incubated 72 hours,The total protein of Hela cell was extracted.Detection COX-2,p-ERK,Bcl-2 by Western blot. To approach which signal transduction were affect in SIN interfere cell proliferation.To observe the general state of Hela cell and the Flow Cytometer were used to detect apoptosis of Hela cell.Results:1.The grow speed of middle and high dose experimental groups was limted.The value OD of these groups were lower than this of control group(P＜0.05);2.The expression of COX-2 in middle and high dose experimental groups were decrease,compare with control group(P＜0.05);3.The expression of p-ERK in high dose experimental groups was decrease, compare with control group(P＜0.05).But not decrease in low dose group.4.The expression of Bcl-2 in middle and high dose experimental groups were decrease,compare with control group(P＜0.05) 5.The apoptosis rate were increased in experimental groups,compare with control group(P＜0.05).Conclusion 1.SIN can inhibit the proliferation of Hela cell.2.The increase apoptosis of Hela cell were induced by SIN. The probably reason is Bcl-2 decresae.3.The function of COX-2 and ERK-Bcl-2 signal transduction were decreased after use SIN probably.