Experimental Study Qingdushuan Tied To Hela Cells In Vitro Proliferation And Induce Apoptosis

Purpose: By studying medicine Qingdushuan tied to human Hela cells proliferation and apoptosis in vitro, and to explore the mechanism of cervical cancer prevention Qingdushuan bolt from the cellular level.Methods:1. Medicine Qingdushuan bolt purification: chloroform to medicine Qingdushuan bolt extract the active ingredients2. CCK-8 assay to detect Hela cells in vitro growth inhibition assay: Influence in medicine Qingdushuan tied to different concentrations of Hela cells 24,48,72 h, and the establishment of the control group and the control group of insurance baofukangshuan by CCK-8 assay the drug on cell growth and proliferation, detected the best point time and the best concentration and detected the OD.3. Mitochondrial membrane potential to detect apoptosis experiments: Use of JC-1 mitochondrial membrane potential assay kit mitochondrial activity of different concentrations medicine Qingdushuan bolt action Hela cells, thereby further understand Chinese medicine Qingdushuan bolt induced cell apoptosis pathway.4. Apoptosis experiments : utilization annexin-V / PI kit fluorescent probes to detected different concentrations of drugs Hela Hela cellsResults: High-dose Qingdushuan tied at 24 h when Hela cells significantly inhibited proliferation and induced apoptosis, and low dose group compared with the control group and Qingdushuan bolt respectively, the difference was statistically significant(P<0.05), disinfection bolt and bolt Baofukangshuan control group, no significant difference(P> 0.05).