Experimental Study On Induction Of Apoptosis Of Cervical Cancer Hela Cells And Clinical Observation On Cin By Er Huang San

Objective:1.The aim of this study was to investigate the mechanism of ER HUANG SAN on cell proliferation and cell apoptosis of cervical cancer Hela cells.2. Observating the clinical effect of Er Huang San in treating CIN.Method:1.Hela cells were cultured in vitro.MTT assay was used to determine the proliferation of Hela cells;Transmission eleetron was used to observe the morphologie alalteration of Hela cells treated with E HUANG SAN;Flows the type cell technique law examinate cell weak perishing rate;Immunity group reduction Examinate p53 protein expression level in the cell;RT-PCR method examinate mRNA of HPV18 E6 expression level in the cell.2.Take 30 example CIN patient as the object of study, Treats for 3 months using Er Huang San.Through observation clinical symptoms,Cervix of the uterus local circumstances,TCT organization pathology inspection, Compared with treatment around difference.Result:1.Different density Er Huang San has the inhibitory action to the Hela ell,Inhibitory action enhancement along with dosage mark up;Transmission eleetron was used to observe 2.5mg/ml Er Huang San affects the Hela cell 24 hour obvious early time perishes weakly the cell;Flows the type cell technique law examinate cell weak perishing rate enhancement along with dosage mark up;Immunity group reduction find 2.5mg/ml Er Huang San affects the Hela cell 24 hour p53 protein expression enhancement;Er Huang San may decline in the Hela cell the HPV18 E6 mRNA expression.2.Er Huang San to ASCUS,LSIL,the HSIL effectiveness respectively is 75%,78.57%,66.67%;Er Huang San to the CINII total effectiveness 66.67%;Er Huang San to the mild erosion,the moderate erosion,specific weight erosion effectiveness respectively is 75%,85.71,66.67%;The leucorrhea increases, in the leucorrhea the belt blood or the contacting vagina bleeds symptom vanishing rate respectively is few 83.37%,71.42%,60%;Processes various numbers of classes after statistics to occupy statistics significance.Conclusion:1.Er Huang San may suppresses the Hela cell's Multiplication,becomes the density dependence;Er Huang San can induces the Hela cell to perish weakly, possible with to surrender to the state the p53 protein,declines the expression of HPV18 E6 mRNA to concern.2.Er Huang San reduces the cervix of the uterus epidermis heterogeneous type cell andImproves the CIN patient clinical symptoms and the cervix of the uterus local circumstances.