Oviducts Ranae On The Immune Function Of Fatty Acid

The Forest Frog's Oviduct has very high nutritional value and the healthy function.It is straightens up the solid that raw material for medicine,this is in the version of the Chinese Pharmacopoeia(2005 Edition).At present,the important question of the Forest Frog's Oviduct is the drug efficacy material that isn't clear,therefore strengthens the Forest Frog's Oviduct active material basic research is extremely important.The Forest Frog's Oviduct includes about 5～8%fatty acid,discusses that to the mouse lymphocyte multiplication active influence,observes it in the adjustment organism fatty acid constitution change,deter-mined in the Forest Frog's Oviduct the fatty acid ingredient in immunity adjustment is this topic research objective.The paper embarked from the traditional Chinese pharmacology and the Pharm acology two angles,analyzes northeast to produce in the Forest Frog's Oviduct,the fatty acid ingredient type and the content,and to the lymphocyte function influence function, for further discussed the Forest Frog's Oviduct the clinical practice value to provide the reliable scientific basis.Objective:Studies in the Forest Frog's Oviduct that the fatty acid ingredient type and the comparative content;confirms the influence of the immune function.Method:1.Uses the saponified law to withdraw in the Forest Frog's Oviduct the fatty acid ingredient,determines its composition and the comparative content using the gas chromatography technology;2. Uses the single factor inspection experiment,the contrast different dosage,the different extraction method solution creates around the immunity low model to the different kind of mouse that the body weight,the internal organs index and in vivo fat acid content influence function,the determination best Pharmacology experiment plan.3.Presses the preferred plan development pharmacology experiment,uses the saponified law to carry on in the mouse spleen,the thymus and the blood the fat acid content analysis;Uses flows the type cell technique determination mouse spleen T lymphocyte subgroup content;ELISA in law determination spleen T lymphocyte IL-2 content;Using statistical method analytical investigation result.Result:1.The 25 kind of fatty acid of the Forest Frog's Oviduct contents are 812.89mg/100g,EPA and DHA total content are 17.15%,is close with the fish oil.2. Mice make around the mold the comparison,in the model mouse blood,the spleen,the thymus fat acid content reduce obviously;After the model mouse fills the stomach of three dosage the Forest Frog's Oviduct perquisite solution,in the blood,the spleen,the thymus fat acid content has the significance ascension;3.Looked from two immunology target result that,breathes out the frog oil to be able obviously to enhance in the low immunity mouse spleen T lymphocyte CD3,CD4,the CD4/CD8 level and the IL-2 content.Conclusionn:The fatty acid of the Forest Frog's Oviduct has the ingredient of adjustment immunity low mouse in vivo fat acid content and the immune function.