The Repair Function Of Procyanidins On Trans Fatty Acid-induced Immune Injury In Mice

Objective:1.To study the repair function of Proantho Cyanidins（Proantho Cyanidins PC）on the immune injury in mice by Trans fatty acid (Trans fatty acid，TFA).2.To explore the possible mechanism of repair function of Proantho Cyanidinson the immune injury in mice which induced by Trans fatty acid.Method:The mice were randomly grouped for each group of eight:Solvent controlgroup;PC dose group, TFA+low,medium and high doses of PC groups;TFAexposure group.Solvent control group was given deionized water;PC dose groupwas gavaged200mg/kg·bw pc solution for three weeks;The TFA control group wasgiven10ml/kg bw TFA solution by gavage for three weeks;TFA+low,mediumand high doses of PC groups, the three groups were gavaged10ml/kg bw TFAsolution for the first day,and then the mice were fed100mg/kg bw,200mg/kg bw,400mg/kg bw solution for the second day,feeding them like thisway for three weeks.24H after the last administrition,take the blood of eye,the preparation ofserum,and to detect the SOD and MDA.we used the neutral red to detection ofperitoneal phagocytosis.Preparation of spleen cell suspension,detection of NKcell activity ang T cell proliferation were measured by MTT assay;hemolyticplaque assay to detect the number of antibody-forming cells.Calculate the micedelayed type hypersensitivity response by ear swelling;Calculate the coefficientof organ.routine pathological sections,HE staining was used to observe the change of spleen in morphology.Result:1.TFA exposure group compared with the solvent control group wasdecreased(P<0.05)；the weight gain of TFA+PC group is higher than TFA exposuregroups(P<0.05).2.TFA exposure group relative to the solvent control group,thespleen,thymus organ coefficient were decreased(P<0.05);both the spleen,thymusorgan coefficient of TFA+middle and high-dose PC groups were higher than TFAexposure group(P<0.05); the thymus organ coefficient with PC doses in adose-response relationship.3.Spleen histopathology indicates that:Solvent control group:spleen tissuecapsule was the complete and smooth,the splenic evenly distributed,red and whitepulp clear boundaries,the splenic cord and spleen sinus of red pulp arrangednormal,white pulp germinal centers clearly visible,with uniform distribution oflymphocytes;TFA exposed group:The structure of spleen corpuscles was damage,thesplenic cord and splenic sinusoid were disorder;arterial vascular wall of white pulpwas thicker and red pulp was more thinner and narrower.The central artery and Vesselwall get thick，the Lumen get thin,lymphocytes decreased significantly;TFA+PCgroup:200-400dose PC group Compared to the solvent control group,there was nomore difference,with the increase of the PC dose,the improvement is moreobvious.Indicating that a certain dose PC can repair the splenic tissue injury from TFAinduced,so as to achieve the protection of immune organs. PC dose group:the spleenmorphology compared with the control group almost no change.4.TFA exposure group Compared to the control group of mice with NK cellactivity was reduced(P<0.05);Compared to the TFA exposure group, NK cell activityof TFA+middle and high-dose PC group were higher. There is significant differencebetween them (P<0.05),and a dose-effect relationship.5.TFA exposed group compared to the solvent control group, the neutral redphagocytosis of the peritoneal macrophage was significantly lower (P<0.05);Compared with the TFA exposed group, the neutral red phagocytosis of the peritoneal macrophage of TFA+medium and high-doses PC groups was significantly higher(P<0.05).6.The numbers of antibody-forming cells TFA exposed group compared to thesolvent control group,was lower(P<0.05);TFA+middle and high-dose PC groupscompared to the TFA exposed group,the number of antibody-forming cells werehigher (P<0.05).7. TFA exposed group compared to the solvent control group, was reduced(P<0.05);The lymphocyte proliferation from TFA+middle and high-dose PC groupswere more increase than the TFA exposed group.There is significant differencebetween them.(P<0.05).8.TFA exposure group with the control group. the ear swelling of the left and rightears flaps may reduce (P<0.05);The TFA+PC dose groups compared to TFA exposedgroup were increased.(P<0.05).9.Compared with the control group, TFA exposed to the serum SOD activity,MDA content was significantly higher (P<0.05);Compared with the TFA exposedgroup,the serum SOD activity of TFA+middle and high-dose PC protection groupswere increased,the content of MDA were decreased (P<0.05); and dose-effectrelationship.Conclusion:1.200-400mg/kg·bw dose of PC can repair the immune dysfunction in mice causedby TFA;2.PC has a protective effect on TFA induced immune dysfunction,the protectivemechanism could be oxidative damage.