Bzx Phlegm Medicine A Experimental Study On Effects Of Thyroid Cells To Apoptosis

ObjectiveThyromegaly exists abroadly in the thyroid diseases,it is the reason of thyroid cell proliferation＞cell apoptosis.The treatment of thyromegaly in TCM had a history of several thousand years,the principle of removing phlegm and resovling mass is the basic treatment and have an effective cure for thyromegaly in clinic.It contains iodine-rich herbs and None rich iodine herbs.Now we know little about the mechanism of None rich iodine herbs and it needs further research.Through investigating the effect of removing phlegm and resovling mass Herbs Jiazhongxiao (including Spica prunellae and so on)on the apoptosis and expressions of bax,bcl-2 in FRTL cells,to explore the therapeutic mechanism of thyromegaly of the removing phlegm and resovling mass Herbs.And it is supposed to provide some basic research for the therapy of thyromegaly in Traditional Chinese Medicine.MethodsRats'Thyroid FRTL cells was interfered by Serum of Chinese Herbs of Removing phlegm and Resovling mass in vitro.Determined the effect of its herbs-containing serum on the apoptosis of FRTL cells.The cells were divided into six groups:A: normal group;B:blank group(containing 1%non-herbs serum);Tested groups are C,D,E:low,middling and high dose groups(containing1%,5%,10%herbs serum respectively);F:postived control group(GSHNO induce apoptosis group containing 2%GSHNO).The protein and mRNA were extracted from each group's cells separately.mRNA expressions of Bax and Bcl-2 were detected with real-time quantitative RT-PCR.The protein expressions were tested by Western Blot.We used SPSS11.5 to analyze the data.The main statistic methods contained ANOVA,LSD-t,Dunnett-t and Tamhane Tests.ResultsThe mRNA and protein expressions of Bax increased distinctly in the high dose group and middling group.The ratio of Bax/Bcl-2 all increased in the three Chinese herbs groups,but remarkably only in the ZJ group(P＜0.05).Bax mRNA and protein expressions was obvious in the GSHNO group.ConclusionThis study showed that Chinese Herbs of removing phlegm and resovling mass can promote the apoptosis of thyroid cells and increase the pro-apoptosis gene Bax, which may be related to the mechanism of Jiazhongxiao's therapeutical effect on thyromegaly,and gene Bax may be the key point.