The Theory Of Qi And Meridians Of The Heart With Aging In Rats Of D-galactose Aging Model Mice

Objective Succeed in duplicating little old and feeble mouse's model and regarded hiding the starting point like the heart characteristic as, make the collaterals and subcollaterals as foundation,establishing the method of reinforcing Qi and communicating collaterals, use the treatment of reinforcing Qi and communicating collaterals, probe into heart hide like the relation with aging, announce tentatively that stores it in the scientific intension of the anti-aging respect like the heart.Methods 1. This experiment sets up 6 groups: Plant weight 20±1 grams of 72 little mouse, 3 months old Kunming, male and female half and half, divided cage into to be ordinary to raise 6 day so as to conform to environment, divided into 6 groups at random after observe and has not unusually reactions, among them 12 were regarded as the normal group, other 60 were divided into model group, reinforcing Qi group, communicating collaterals group, reinforcing Qi and communicating collaterals group and Vitamin E group respectively. We adopted each little mouse every day neck dorsal, 1g of hypodermic injection, to model group and medicine group subsequently; 1%D -Galactose 0.1ml caused subnormal temperature old and feeble model, normal group little mouse every day neck dorsal hypodermic injection physiological saline 0.1ml, injected for 6 weeks continuously. While injecting D - Galactose from the 7th day, every medicine group began to give medicine, adopted and irritated the stomach method and gave medicine, the reinforcing Qi group presses of 3. 25g (kg·d)^-1 offered reinforcing Qi medicine to fry unguentum mix liquid (0.2ml); the communicating collaterals group presses of 5g (kg ? d)^-1 offered communicating collaterals medicine to fry unguentum mix liquid (0.2ml); The reinforcing Qi and communicating collaterals group of benefit presses of 6. 5 g(kg ? d)^-1 was offered reinforcing Qi and communicating collaterals medicine to fry unguentum to mix the liquid (0. 2ml); The Western medicine group pressed of 50 mg (kg ? d)^-1 mixes, hung the liquid (0. 2ml) the Vitamin E; Once a day , for five weeks in succession. Normal group and model group offered, etc. the physiological saline of capacity irritated the stomach, Once a day, for five weeks in succession. Little mouse were feed in free diet of experiments. 2.Medicine : Reinforcing Qi medicine was made up of sun-dried ginseng 15g and cassia twig 10g, concentrated this side as traditional Chinese medicine preparation containing crude medicine 0. 325g/ml, people mouse weight it lasted amounts of medicine given of mouse little to be 0. 2ml / only /day; Communicating collaterals medicine was made up of leech 10g, ground beetle's worm 5g, earthworm 10g and cassia twig 10g, concentrated this side as traditional Chinese medicine preparation containing crude medicine 0. 5g/ml, it lasted amounts of medicine given of mouse little to be 0.2ml according to people mouse weight / only /day; Reinforcing Qi and communicating collaterals medicine was made up of sun-dried ginseng 15g, leech 10g, ground beetle's worm 5g, earthworm 10g and cassia twig 10g, this it concentrate side it lasted amount of medicine given 0.2ml of mouses little to traditional Chinese medicine preparations containing crude medicine 0. 65g/ml according to people mouse weight /only /day; Worthy of become mix liquid will be hung Vitamin E glue pill, last amount of medicine given 0. 2ml of little mouse according to people mouse weight 0.2ml / only /day; Normal group, model group offered, etc. the physiological saline of capacity irritated the stomach, the number of times and course of treatment are the same as medicine groups. 3.Then measured the benefit of medicine separately, reinforcing Qi medicine, communicating collaterals medicine, reinforcing Qi and communicating collaterals medicine and Vitamin E to old and feeble model little mouse such as the influence in ethology, SOD, MDA, the ultramicrostructure of cardiac muscle cell.Results 1. Reinforcing Qi medicine, communicating collaterals medicine, reinforcing Qi and communicating collaterals medicine obviously expanded the function of ethology to old and feeble model little mouse, reinforcing Qi and communicating collaterals medicine group result superior to communicating collaterals group, reinforcing Qi and communicating collaterals group and Vitamin E group obviously among them. Ethology function was improvement; 2. Reinforcing Qi medicine, communicating collaterals medicine, reinforcing Qi and communicating collaterals medicine and Vitamin E can raise old and feeble model little mouse serum hitted SOD activity and gegraded the content of MDA effectively, and had the best result with the reinforcing Qi and communicating collaterals group of benefit. 3.Vitamin E group, reinforcing Qi group, communicating collaterals group and reinforcing Qi and communicating collaterals group's change were improved to little mouse cardiac muscle cell pathology of ultramicrostructure old and feeble to some extent, but the reinforcing Qi and communicating collaterals group of benefit were obviously superior to every other group.Conclusions Model group little mouse serum hit the content of SOD reduce obviously than normal group, proved succeed in duplicating old and feeble animal model already. The treatment of reinforcing Qi and communicating collaterals could improve ethology effectively, improve SOD activity and degrade the content of MDA in the serum, change obvious improvement function to the pathology of the cardiac muscle cell ultramicrostructure, thus get the result of slowing aging.