| Common Anemarrhena Rhizome was firstly recorded on the"Shen Nong Ben Cao Jing", which was listed as a middle one. It is the dry rhizome of Anemarrhena asphodeloides Bge., belonging to Liliaceae. It is commonly used on clinic as the heat-clearing and fire-purging drug and is widely distributed in the North China, Korea and Mongolia. The quality is different because there are many resouses, such as different places of products and collection periods. The quality study of it was mainly concerned on several constituents, such as sarsasapogenin and mangiferin. The whole quality character couldn't be reflected by these analyses.Meanwile, there was no standard of cultivation technique when producing. So the quality of it is difficult to guarantee.This article is mainly to solve the above problems and analyse quite a lot of samples which were collected in the North China by wild investigation, field experiment and laboratory analysis. In order to identify the quality character and the influencing factors on the contents and proportion of constituents, and the relationship between the contents and the climatic factors, we do the research on the quality character of the wild growth and the cultivated ones by studing the multiple constituents determination and HPLC fingerprint.(1)HPLC–ELSD fingerprint was set up. The four contents neo-mangigein, magigein, timosaponin C, timosasponin AⅢare identified.The semblance of the two fingerprint are all above 0.97. There are some differences between two regions.(2)The relationship between the contents and climatic factors was analysed. It shows that the sarsasapogenin, mangigerin, neo-mangigein, timosaponin C timosasponin AⅢand crude fat all have not significant difference(P>0.05)with year sam, July sam, annual precipitation, evaporation capacity, sunshine duration, frost-free period. The crude protein has significant difference(P<0.05)with July sam and annual preipitation. The polycose has significant difference(P<0.05)with annual preipitation and frost-free period, and has most significant difference(P<0.01)with July sam.(3)Investigate the influnce of cultivate measures to constituents. the compound fertilizer and farmyard manure have significant influence to the active contents.There is no significant influence to the active contents in different margins. The content of crude fat is significant different(P<0.05) under different manure match. Rational use N,P,K and proper match are good for accumulation of crude fat and protein and also for increasing the outcome.(4)Reveal the content character of microelements and investigate the influence of enviroment and cultivated measure to the content of constituents. The content of Mn has significant direct correlation(P<0.01)with evaporation capacity. The content of Mn has inverse correlation with year sam, July sam, sunshine duration and frost-free period. The content of P has no significant relationship with the six factors(P>0.05).It has direct correlation with evaporation capacity and inverse correlation with the others.The content of Na+ with different growth time limit are significant different(P<0.05),and the content is highest of the fourth age. The content of K+ are significant differen(tP<0.01) between each handling in potash manure. It is good for the accumulation of K when consperg three times each year.(5)Estimate the range of content of chemical constituent. The content range of the crude fat, crude protein, total polysaccharide, sarsasapogenin, Anemasaponin C, AnemasaponinAⅢ, neomangiferin, mangiferin are respectively 1.27～1.71%, 6.91~9.42%, 13.8~18.4%, 0.87~1.89%, 1.05~1.97%, 0.34~0.99%, 0.80~1.80%, 0.45~1.15%.The distinctness of this article have the following aspects:(1) The methods of extracting contents of crude fat and crude protein were set up.(2) The HPLC-ELSD fingerprint was set up. The four active compounds were identified.(3)Determine the contents of ten kinds of microelements. Reveal the quality character from the angle of microelements. |
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