Fine Column To Add Its Belong To The Same Plant Active Ingredient

There are about 37 kinds of Acanthopanax Miq.plants of Araliaceae in the world,which mainly being distributed in China,Korea and Japan of Asia.The root bark,commonly called "Cortex Acanthopanacis",is a common traditional Chinese drug,which have efficiencies of invigoration, antirheumatism,antifatigue and stimulate circulation to end stasis.It is used for treat the rheumatism pain,waist and knee aching etc.A mass of Acanthopanax Miq.plants grow in our country,about 26 kinds and 18 variety,and the root bark of Acanthopanax gracilistylus W.W.Smith is recorded in Chinese Pharmacopeia.There are 11 kinds,3 variety and 3 form in Korea,and the root bark of Acanthopanax sessiliflorum Seemann is recorded in Korea Pharmacopeia.Just for the further development and utilization of these plants and search more active ingredients,we research on the active ingredients including triterpene,diterpene and voltatile oil from the leaves of Acanthopanax gracilistylus W.W.Smith and other Acanthopanax Miq.plants.Triterpene from Acanthopanax Miq.plants has much bioactivity. To develop new anti-HMGB1 drugs,we research on the triterpene components of Acanthopanax gracilistylus W.W.Smith leaves collected from Changsha in Hunan.The hot methanol extract was primarily separated by Diaion HP-20P CC.Then the 100%and 80%portions were orderly separated by Silica gel CC,Sephadex LH-20 CC,TLC, recrystallization,and finally isolated six components.Multiple spectrum techniques,like EI-MS,HR-MS,1D-NMR,2D-NMR,IR were used for qualitative analysis of compound 1～5.After compared with the data of known compounds,three lupane triterpene components were identified. There were acankoreanogenin A(compound 1,2 and 4),acankoreoside B (compound 3)and acankoreoside A(compound 5),which compound 3 was isolated from this plant for first time.Compound 6 were the mixture of ester by GC-MS analysis.Anti-HMGB1 activity test was made,the results showed acankoreanogenin A and acankoreoside A both have some antiphlogistic activity,and acankoreanogenin A has a good catastaltic effect to HMGB1.Maybe it will be a new anti-HMGB1 drug in future. The conclusion of study would provide gist for the further development and utilization of Acanthopanax gracilistylus W.W.Smith.The research showed diterpene,one of the active ingredients from Cortex Acanthopanacis,has antiphlogistic activity.The mothed of Silica gel CC and TLC was repeatedly used to isolate the ether extract of root bark of Acanthopanax gracilistylus W.W.Smith,and two components were obtained.By identified,compound 7 was acanthoic acid and compound 8 was kaurenoic acid.RP-HPLC method was established for quantitative analysis of two diterpenes for first time.The best condition of HPLC:wavelength is 207nm,temp is 30℃,flow rate is 1ml/min, mobile phase is CH₃CN-H₂O(0～60min CH₃CN 10%→100%,60～70min CH₃CN 100%).Through the methodology test,the method was simple and fast to use,and could gain good accuracy.The diterpene compounds from 8 kinds of Acanthopanax Miq.plants were quantitative analysed by this RP-HPLC method.The results would surely aid to construct the new standard for the identification and quality control of Acanthopanax Miq. plants.Most of voltatile oil from Acanthopanax Miq.plants has antiseptic, antiphlogistic,apophlegmatic and antitussive activity.The method of water-steam distillation was used to extract the essential oils from 8 kinds of Acanthopanax Miq.plants,and the chemical components were identified by GC-MS-DS techniques.Compared with these essential oils, and the common compounds wereα-pinene,β-pinene,spathulenol and n-hexadecanoic acid.The results would provide investigative basis for further development of Acanthopanax Miq.plants.The results of this paper has important effect to choose anti-HMGB1 drug.And it will enrich natural products database,and provide important theoretical foundation for identification of active ingredients,systematics of phytochemistry and new drug screening.So it will has referred value on developing and exploitaging our country's natural products resource.