The Actinomycetes I03a-02842 Of The Screening, Separation And Purification Of The Active Product And The Minor Components Of Sansanmycins,

The current work included two parts. One is the screening of anti-tuberculosismicroorganism I03A-02842 and the isolation and purification of its fermentationproducts. The other is the isolation, purification and structural identification ofSansanmycin D, E.The DNA topoisomeraseⅡof bacteria had two subunits: A and B. Subunit A is thetarget of quinolone drugs. Subunit B is a new kind of screening target for anti-TBdrugs. The screening model targeting subunit B permitted obtaining active compoundswithout cross resistance with quinolone drugs. Professor Xiao had found a series ofI03A microorganism with the screening mode. The strain I03A-02842 were obtainedfrom the series of I03A microorganism by means of macroporous adsorption resinchromatography, paper chromatography and determination of antibacterial activity.Fermentation broth was filtered and applied sequentially on the columns ofmacroporous adsorption resin and cation exchange resin. The effluent and eluent wereobtained from the cation exchange resin column. By means of extraction with ethylacetate (PH=7) and preparative thin layer chromatography, two active compounds, Aand B, were obtained from the effluent. The analysis of LC-MS showed the molecularweight of the compound A (612)and B (624). But their structures were not identifieddue to the little content. The eluent included water-soluble components which had theactivity against M. phlei and gram positive bacteria. SP-Sephadex-C-25 and ODScolumn chromatography were used to isolate and purify the eluent, but enough samplewas not obtained to be used for the analysis and structural identification.Sansanmycin D and E were obtained by macroporous adsorption resin and ODScolumn chromatography as well as preparative HPLC. The structure of SansanmycinD was established by NMR spectral analysis. It proved to be the same as that ofMureidomycin A. The structure of Sansanmycin E was deduced by analyzing itsESI-MS, ESI-MS/MS, physical-chemical properties, ultraviolet spectrum.Sansanmycin D and E had good activity against pseudomonas aeruginosa andM. phlei.