Self-prescription Of Acute Gouty Arthritis Of The Knee Synovial Tissue Of A Rabbit Model Of Interleukin 8 (il-8) And Cyclooxygenase 2 (cox-2) Affect Experimental Research

ObjeCt. TO determine whether Chinese herbs leads to changes in COX-2mRNA levels for molecules and IL-8 in synovium in an experimental model. Methods. We created the model of monosodium urate crystal-induced rabbit arthritis according to Mc Carty DJ. Total-RNA was isolated from synovium at 2h. COX-2 was assessed by semiquantitative reverse transcription pol ymerase chain reaction. PCR products were separated in a 2% agarose gel. The photographs were analyzed using the Kodak. IL-8 was asseeeed by ELISA. Results. The levels of COX-2 and IL-8 in Chinese herbs group are significantly lower than that in model-group (p＜0.05). Conclusions. chinese herbs could significantly reduce the level of COX-2 and IL-8 in synovium of MSU induced rabbit actue arthritis and had better preventing and curing effect.