Effective Parts Of The New Drugs Gall Bladder Shu

Cholecystitis is an ordinary disease of digestive system, TCM has its own characteristic and superiority in treating it. The research of diminishing and repairing gallbladder is very active.Some new drugs are put to the market,but the investigation of drug's clinical property shows that these drug's curative effects are not good.The purpose of this project is to developa safety, effective medicament that can prevent and cure the disease.The article is the mainly part of "The researching of new drug from Gardenia jasminoides Ellis".In the course of study,according to the technical claim of examination and approval method of new drug which was issued byState Drug Demonstration,technological process,quality standard and pharmacodynamics related to valid part of material were studied in order. Weinvestigated the preparation of the tablet, compared three different extract technology of Gardenia.The optimum extraction process was investigated by L₉(3⁴) orthogonal experimental design.The concentration ethanol,amount of ethanol,extraction time and extraction times were the four factors in the experiment. The optimum extraction process was: extracted with 70% ethanol for 3 times,with the solvent volume 8 times amount and extraction time 1.5heach time.To select the optimum purification process of all iridoidsides from Gardenia by macroporous resin, absorption test and deabsorption test were carried out to screen the best macroporous resin for all iridoidsides and to study the absorption characteristics and elutive parameters of the process.Themacroporous resin HPD100 possessed strong absorption ability and an easy de-absorption property.The optimum absorption condition was A₂B₃C₂, namelythe weight ratio of solution and raw material 3:1, diameter:height of the chromatography column 1:7, the weight ratio of raw material and resins 1:2. We select proper diluents, adhesives and wetting agent through experiment and make the extract into tablet.In the quality control study, TLC was used for the identification of Gardenia.This method was simple, rapid and with a good reappearance, and available control of the preparation.The quality of all iridoidsides was determined by UV. The linear range of Geniposide was 0.00512mg/ml-0.04096mg/ml,R=0.9994.In the extract, the average recovery was 97.87% and RSD was 1.14%.In the tablet, the average recovery was 98.11% and RSD was 0.63%. The quality of Geniposide was determined by HPLC. The linear range of Geniposide was 0.1024-0.512μg, R=0.9999 .In the extract, the average recovery was 98.13% and RSD was 0.67%.In the tablet, the averagerecovery was 98.56% and RSD was 1.58%..The method is simple and accurate. It can be used for quality control of Danshu Pian.We confirm the effective composition of Gardenia jasminiodes Ellis by pharmacology experiments.We confirm its therapeutic effect of the iridoids from Gardenia through the study of the influence of it on bile and...