Low-intensity Helium-neon Laser Irradiation On The Rate Of Red Blood

In this article, the ratio of mouse's erythrocyte hemolysis due to low-intensity He-Ne laser irradiation is studied in experimental, laser power and irradiation time is different. The bio-stimulation effect and influencing factor of erythrocyte hemolysis is analyzed.The mouse's 10% fresh erythrocyte suspension is the object to be observed, sodium heparin solution is the anticoagulant. Setting up laser power, spot size and time to be irradiated, Terminate irradiation and lay-aside the sample in refrigerator a period of time, the different ratio of erythrocyte hemolysis of irradiation group and control group with time-variation will be investigated during the standing time (0—24h).Experimental result indicated that low-intensity He-Ne laser irradiation enhances the ratio of erythrocyte hemolysis. So that the erythrocyte is the target cell and sensitive to the laser irradiation, it could be the effective target model in researching the bio-stimulation effect of low-intensity laser irradiation. There are four significant characteristics about the biological effect. First, it is kind of delay effect after laser irradiation. At the end of irradiation, no erythrocyte hemolysis is detected. While conserved for 24 hours significant difference exists between the two groups. On the other hand, hemylosis is positive correlation with laser power when the irradiate time is similar. Thirdly, hemylosis is positive correlation with irradiation time when the laser power is similar. Finally, there is marked individual difference of the reaction.The result of this paper will provide reference meaning of the study of mechanism about photo-blood interaction and low-intensity laser biological effect, and low-intensity laser clinical application. The method of this paper will supply referential model about the study of low-intensity laser biological effect.