Weak Laser And LED Light On The Isolated Rabbit Erythrocyte Impact Experiments

Bio-effect of low intensity laser irradiation and LED has obtained curative effects in many fields and has a broad prospect especially in clinical medicine, which has attracted great interest at home and abroad. However, there are still numerous disputes on its mechanism, which hasn't reached a unified understanding. Some questions should be solved based on the inadequate basic researches.In this paper, the hemolytic effect of rabbit erythrocyte in vitro under irradiation of 405nm laser,623nm and 636nm LED was investigated respectively. The rabbit's fresh erythrocyte suspension washed by physiological saline is the object to be observed. The irradiation power of laser and LED is properly set up. The hemolytic rate of erythrocyte suspension is examined in 24 hours after irradiation.Experimental results showed that the hemolytic rate of experimental group of 623nm and 636nm LED was higher significantly than that of the placebo group, and increased with the irradiation power under certain conditions. While the hemolytic rate of 405nm experimental group had no obvious difference from the placebo group.The hemolytic effect of 405,532,623,632.8,636 and 660nm light is compared. The results indicate:(1) Red light could enhance the hemolysis of rabbit erythrocyte, while 405nm and 532nm light couldn't. (2) The hemolysis of 632.8nm group is the maximum, while that of 660nm group is the minimum. (3) The effect of red light is time-delayed. (4)The hemolytic rate of different red light is positive correlation with the fluorescence intensity which is emitted by stimulated porphyrin.Through analyzing the experimental results, a new idea about porphyrin is proposed. Porphyrin from the blood would become photosensitive under the irradiation of red light, which causes photochemical reactions. The series of bio-effects play an important role in low intensity laser and LED irradiation treatment. The absorption mechanism of erythrocyte on violet and green light is different from that of red light, which causing the hemolysis difference between them.The results of this paper will provide important experimental support and theoretical guidance to the further study of mechanism about photo-blood interaction, low-intensity laser and LED treatment.