Ganyanlin Extract Liver Pharmacodynamic Study

GANYANLING(GYL) extracts is a compound Traditional Chinese Medicine including radix astrgadli, radix glycyrrhizae, radix bupleurum, fructus corni, fructus citri sarcodactylis, radix angelicae sinensis, fructus salvaiae miltiorrhizae, schisandrae chnensis, herba sedi, fructus aurantum, hedyotidis herba ,barbed skullcap herb. According to the literature the single medicine can protect liver from damage. The effects of the compound has little reports so we study the GYL's main pharmacodynamics action and its' mechanism .From the results, GYL could prevent liver from acute damage induced by CCl₄, D-Gal, ConA and TAA. It decreased the serum ALT, AST activities in acute hepatic mice and displayd the effects in dose-dependent manner. In chronic hepatic injured experiments, GYL also obviously lowered hepatic cirrhosis rat the serum LN, HA, PⅢP contents and the liver homogenate HYP contents. From the pathologic section, compared with model group, all groups of GYL improved the rat hepatic phathology. Rats hepatic cell necrosis, lipid degeneration, liver fibrosis were all abated. In receding jaudice and normalizing gallbladder experiments, GYL had no obvious effect. In immunology experiments, GYL enhanced phagocyte function and accelarated carbon clearance in immunodepression mice induced by Hydrocortisone(HY). At the same time GYL stimulated antibody forming and increased hymolytic OD in hypoimmunity mice induced by Cyclophosphamide(CTX). In lymphocyte proliferation experiments of ConA stimulated mice, GYL regulated cell immunity according to its concentration . GYL promoted proliferation in lower dose and restrained the proliferation in higher dose. In normal mice lymphocyte proliferation experiments GYL stimulated lymphocyte proliferation in a increasing dose manner. The mechanism showed that it lowered liver homogenate MDA contents in acute liver damage experiment. It also lowered serum MDA, NO contents and raise serum SOD level in hepatic cirrhosis rats. It showed that GYL had the effects of antilipid-peroxidation. GYL also inhabited serum TC and TG in liver fibrosis rats and raised serum TC contents in hepatic cirrhosis rats. It showed that GYL could regulate lipid metabolism. At the same time, GYL could reduce serum AKP,γ-GT contents and raise the contents of CHE and A/G ratio in hepatic cirrhosis rats. The results showed that GYL could improve hepatic function.Above all, GYL can protect liver. It' s mechanism maybe related to it' s antilipid-oxidation, regulation of immunity and lipid metabolism, and improvement of liver function.