Experimental Study Of Non-traumatic Femoral Head Necrosis Femoral Head The Local Vegfmrna With Bmp-2 Expression

Objective To explore the change and its significance of the expression of vascular en-dothelial growth factor (VEGF) messenger RNA and bone morphogenic protein-2 ( BMP -2) of the local femoral head in the nontraumatic osteonecrosis of femoral head (NONFH). Methods Collect 16 samples of femoral heads of NONFH as the experimental group and fresh 10 samples of femoral heads of femoral neck fracture as control group, overall examples were collected from total articular replacement arthroplasty. The samples are splitted in coronal plane and get one bone block from necrosis area and another one from healthy area, make them into microtome section after the process of immobility and decalcification. Observe their pathological change by using optical microscope and electron microscope and detect the expression of BMP - 2 and VEGFmRNA in femoral head through making use of immunohis-tochemistry and in — situ hybridization technique. Results The organization structure of experimental group is disorganized, cracked and the bone trabecula is rarefactive and non- intact and there are a great number of empty lacuna in bone trabecula. While there is the reverse situation in the control group . The intensity and area of positive expression of BMP -2 and VEGFmRNA in femoral head of the experimental group are lower than that of control group obviously. The result shows statistical significance (P<0. 05). Conclusion The decrease of expression of BMP - 2 and VEGFmRNA in femoral head of NONFH is important reason that affect its rehabilitation and reconstruction .