P16 ~ (ink4a), P14 ~ (arf) Protein And Cervical Squamous Cell Carcinoma Of The Formation And Progress Of The Relationship

Objective:To study the expressions and clinical significances of p16INK4A and p14ARF proteins in squamous carcinoma of the cervix(SCC),cervical intraepithelial neoplasia(CIN) and normal cervix samples.Methods:The expressions of p16INK4A and p14ARF proteins were detected in 95 SCC samples and 80 CIN samples by immunohistochemistry (S-P method),and 45 normal cervix samples as control.Results:The portions of p16INK4A and p14ARF–positive samples increase in the following row: normal cervix - CINⅠ- CINⅡ- CINⅢ-SCC,which had significant differences (P1=0.000,P2=0.000).The positive correlations were found between the p16INK4A and the p14ARF expressions in consecutive cervical lesions (r=1).Of the 95 samples analyzed from SCC patients,the positive rates of p16INK4A and p14ARF expressions in the groups of clinical stageⅡ(98.3% and 96.6%) were higher than that in the groups of clinical stageⅠ(83.8% and 78.4%).The expressions of p16INK4A and p14ARF were closely related to clinical stage (P1=0.025,P2=0.013).We also noticed a positive correlations between the p16INK4A expression,p14ARF expression and histological grading in SCC (r=1).The positive expression rates of p16INK4A and p14ARF were not associated with the lymph node metastasis (P>0.05).Conclusion: The positive expressions of p16INK4A and p14ARF were closely related to consecutive cervical lesions. P16INK4A and p14ARF might play roles in the formation and progression of cervical cancer.The expression levels of p16INK4A and p14ARF might be referred as markers of squamous carcinoma of the cervix.