Characteristics Of The Effective Compounds In Wild Chrysanthemum

1.ObjectivesFlos Chrysanthemi Indici is dried capitulum of Chrysanthemum indicum L.. Total flavonoids as pharmacological active constituents have many activitives such as removing toxic heat,lowering the blood pressure,antivirus and Restraining platelet aggregation. Flos Chrysanthemi Indici is leading medicable ingredient of Jing Ju compound preparation,But we don't know active chemical components of Flos Chrysanthemi Indici on antivirus by far. On the basis of the research on the chemical constituents of the effective fraction ,The purpose of our study is to establish the base for finding out a contact with chemical constituents and pharmacological activity further.2.Methods(1)Research method1) Study on the technics of extraction and concentration of total flavoninds with orthogonal test method.2) Study on the isolation and purification of chemical components by a few column chromatogram.3) Study on the structures of compounds on the basis of modern spectrum and MS method.(2) Technique lineWater extraction Concertrating the total flavoninds Confirming the effective fraction Isolating the chemical compounds from the effective fraction Identifying the structures of compounds.3.ContentsOur studies included four parts as follows: (1)The documents about the present research situation in Chinese medicine Flos Chrysanthemi Indici were summed up and analyzed.A review on the research progress of Flos Chrysanthemi Indici in chemical constituents and paharmacological activities was presented.(2) Study on the technics of extraction and concertration of total flavoninds.(3) The systemic study on the chemical constituents of the effective fraction.(4)Summarise the researching result,the academic meaning and valule of the researching results.4.Results(1) Taking the content of total flavonoids as observation index, we searched the effective fraction of water extract from Flos Chrysanthemi Indici .we have studied the extracti on processs by the L9(34) orthogonal design. The best technology is water as the extra cting solvent ,repeat 3 times, every time add 10,10,10 times water respectively and the extracting lasts 50,40,30min each time respectively.The liquid water-extration and alcohol-precipitation(80%ethanol) of Flos Chrysanthemi Indici was treated with a column of macroporous resin and eluted with distilled water,10%ethanol and 30%ethanol.The total flavonoids were enriched and purified in the eluent of 30%ethanol.(2) The chemical constituents of the effective fraction were investigated intensively. 13 compounds were isolated and purified by silica gel,polyamide,sephadex LH-20 column chromatogram. On basis of their spectral data,11of them were elucidate ed as chrysantetriolA,chrysanthetetrol,isoquercitrin,luteolin-7-O-β-D-glucoside, cosmosiin, linarin, eriodictyol, luteolin, quercetin, zhebeiresinol, daucosterol. Among them, chrysantetriolA and chrysanthetetrol are new compounds , isoquercitrin,eriodictyol and zhebeiresinol were obtained from Flos Chrysanthemi Indici for the fist time.5.Conlusions and Creative points(1) The enrichment and purification procedures of total flavonoids from Flos Chrysanthemi Indici with AB-8 macroporous resin absorption were established for the first timeThe concertrating processs have many virtues: with good adsorbability and small deadsorbability as well as low cost, little quantity organic solvent, the process is scientific and simple,the method is stabilization and fesible.(2) We have identified 11compounds from water extraction of flos chrysanthemi indici, and established their constituents characteristic preliminarily in sorts :flavonoids- terpenoids-lignans-steroides.On base of it we can supply the evidences for the quality moniter and control of flos chrysanthemi indici with basic feature.(3) Four compounds were obtained form flos chrysanthemi indici for the fist time ,Among them, chrysantetriolA and chrysanthetetrol are new compounds , isoquercitrin and zhebeiresinol were obtained for the fist time.6.Discussion and Expectation:The most of constituents that we have obtained from water extraction of flos chrysanthemi indici are flavonoids,so it's believed that total flavonoids are the active components in the flos chrysanthemi indici are flavonoids.Chrysanthemol which had been obtained from flos chrysanthemi indici previously showed strong anti-inflammatory activity in mice,so it's worth searching anti-inflammatory activity of chrysantetriolA and chrysanthetetrol as new eudesmane-type sesquiterpenes further.To clarify one's esessential characteristic we should evaluate the quality of Chinese traditional medicine with chemical constituents.