| High performance capillary electrophoresis is an efficient and rapid separationtechnology, for the electric field as the driving force, ions or charged particles move inthe capillary, their speed are according to their mobility and/or different partitioncoefficient. Because of the high resolution, low consumption, rapidness, simpleness,capability of anti-interference and anti-pollution, it has been widely used in the fieldssuch as life sciences, biotechnology, drugs analysis, environmental science etc. Thefollowing reports were presented mainly for the application study of CE on the active ingredient analysis in the Chinese drugs. Including two parts:Study I: CE Analysis of Nine Flavonoids in Flos Chrysanthemiand Flos Chrysanthemi IndiciA method of CZE coupled with DAD was developed for determination of nineactive flavonoids including Linarin, Luteolin-7-O-glucoside, Acacetin, Epicatechin,Catechin, Diosmetin, Luteolin, Apigenin and Kaemferol in Flos chrysanthemi and itskindred plant Flos chrysanthemi indici. Researches on extraction efficiency ofultrasonic extraction and reflux extraction, the different concentration of methanol andextration time were implemented. UV detection wavelength, pH and concentration ofthe running buffer, injection time, applied voltage, temperature, et al were researched.Nine conpounds were baseline separated within30min in a60cm length capillary at aseparation voltage of15kV with a running buffer consisting of20mmol/L borate-50mmol/L sodium phosphate (pH9.6). Excellent linearity of Linarin, Luteolin-7-O-glucoside, Acacetin, Epicatechin, Catechin, Diosmetin, Luteolin, Apigenin andKaemferol were observed at concentration of5.0-200,5.0-200,2.5-120,2.5-120,2.5-120,1.0-120,2.5-120,2.5-120and2.5-120mg/L, respectively. The detection limitswere at2.5,2.5,1.0,1.0,1.0,0.5,1.0,0.5and0.5mg/L, respectively.Using this method, three well-known medicinal Flos chrysanthemi samples(Gongju, Hangju and Boju) and three Flos chrysanthemi indici samples from mainproducing regions Anhui and Hubei provinces of China were analyzed. The resultsshowed that the main components of Flos chrysanthemi were Luteolin-7-O-glucoside,Catechin and Apigenin, the main components of Flos chrysanthemi indici were Linarin,Luteolin-7-O-glucoside, Luteolin and Catechin. Linarin presented a high concentration(2060.27±37.48μg/g) in hangju, a relative low concentration (544.85±17.54μg/g) in boju, and it was not found in Gongju. Boju was abundance of Luteolin-7-O-glucoside,and Luteolin, whose content were about2.4,8.7times of Gongju and4.1,3.5times ofHangju, respectively. Apigenin presented a high concentration (2377.40±61.38μg/g) inBoju, but can not be quantited determination in Gongju and Hangju. In Floschrysanthemi indici, Linarin presented the highest concentrations (1694.26-7266.33μg/g), followed by Luteolin-7-O-glucoside (1131.72-1688.60μg/g), Catechin(637.63-1279.96μg/g) and Luteolin (524.32-904.34μg/g). The content of Acacetin,Epicatechin Diosmetin and Kaemferol were low in all the six samples. For the kindredplants from the same geographical origin Bozhou, Flos chrysanthemi indici presentedabundance of Linarin whose content was13.5times of Boju. But Boju was abundanceof Luteolin-7-O-glucoside, Luteolin and Apigenin, about5.5,3.5,25.5times of thekindred plant, respectively.The method is simple, reproducible, and accurate. It is a reliable method forquantitative analysis of flavonoids in Flos chrysanthemi and Flos chrysanthemi indici. Itcould provide some references for the quality control of herbs and theirphytopharmaceuticals.Study II: CE Analysis of Phenolic Compounds in Six ExtractionSolution of Bidens Bipinnata L.To develop a novel method using capillary electrophoresis coupled with DADdetection for simultaneous determination of ten phenolic compounds(Luteolin-7-O-glucoside, Rutin, Quercetin-3-glucoside, Quercetin-3-rhamnoside,Salicylic acid, Luteolin, Kaemferol, Quercetin, Gallic acid and Protocatechuic acid) inBidens Bipinnata L.. Extraction efficiency of above-mentioned compounds using sixdifferent ethanol/water solutions was compared. The pH and concentration of running buffer, applied voltage, temperature, et al were researched. Ten compounds werebaseline separated within16min in a60cm length capillary at a separation voltage of25kV with a running buffer consisting of25mmol/L borate (pH9.6) at214nm.Excellent linearity of Luteolin-7-O-glucoside, Rutin, Quercetin-3-glucoside,Quercetin-3-rhamnoside, Salicylic acid, Luteolin, Kaemferol, Quercetin, Gallic acid andProtocatechuic acid were observed at concentration of5.0-120,5.0-120,5.0-120,5.0-120,1.0-120,2.5-120,5.0-120,2.5-120,2.5-120and2.5-120mg/L, respectively.The detection limits were at2.5,2.5,2.5,2.5,0.5,1.0,2.5,1.0,1.0and1.0mg/Lrespectively. The relative standard deviations (RSDs) of precision were below5.17%,(n=6). The mean recoveries for ten phenolic components in Bidens Bipinnata L. rangedfrom94.4%to105.8%. The proposed method is highly selective and sensitive,reproducible, and suitable for the quality control of ten phenolic compoundscomponents in Bidens Bipinnata L. extraction solutions. |
