Cancer Cells, Breast Disease Samples, Absorption Spectra And Raman Spectroscopy Study

Spectroscopy is an effective method in structure, component and content analysis of substances, due to its high sensitivity. It can diagnose diseases in molecule and cell level from the changes of tissues, body fluids and cells. Compared to other methods, spectroscopic diagnosis is non-destructive, non-invading and high-automatization. In addition, it may be used as a potential tool for diagnosing disease in early stage, particularly for cancers.Spectroscopy has an evident advantage in analysing components and diagnosing cancer. But at present many groups mostly focu on the spectrum reseach in some simple of simple illness, who give priority to analyse the spectrum in tissues, serum, not develop to it of pathological sections. This article reseaches the spectrum in serum, pathological section and tissues of breast disease suffererings. And we give qualitative analysis about absorption spectrum of cancer cells and their culture mediums.This thesis includes four parts, and some important conclusions are drawn:In the first part, the absorption spectra of two type of cancer cells, i.e. CNE and Hela and their culture mediums during the course of growing are measured. The fringe analyses shows that the amino acid's content is higher in culture medium DMEM than in 1640; The tendency of amino acid consumed is almost consistent between CNE and Hela cells, suggesting that the growth rhythms are similar in some degree for different cancer cells. CNE cells consume more amino acid than Hela cells do. And the proportion of each type aromatic amino acids consumed is different, so the specific metabolite are produced.In the 2nd part, we measured the absorption spectra of serum. The results indicate that the absorption peak aroud 279nm in serum spectra is blue-shifted for sufferers due to a relative higher content ratio of tyrosine to tryptophane. In addition, an absorption peak at 414nm appears in the serum spectra, this is probably resulted from the higher hemolysis of most breast patients.We studied the influence of cancer cells on the absorption spectra. The absorption band around 279nm can be deconvoluted into the absorptions of tyrosine about 273nm and tryptophane about 283nm, whose intensities are nonlinear dependant on the quantity of cancer cells. The cancer cells don't destroy the conjugated double bond structure of the aromatic amino acids in protein, but consume unequally the quantity of tyrosine and tryptophane.In the 3rd part, Raman spectroscopic study on breast pathological sections shows that the peaks at 1452cm"1,1527.2cm"\ 1613.04cm"1 are blue-shifted for cancer ones and the relative intensity of 11453/11343^ Ii453/Ii239> I1613/I1525 can also be used to discriminate cancels from normal ones. The dividing line between hyperplasia and cancer cells is 0.993, 1.151, 1.018 respectively. The former is lower and the latter is higher than these values.In the 4th part, the Raman spectroscopy study of peripheral tissues shows that the peak at 1156 cm"1 and 1530 cm"1 from carotinoid C=C bond is more intense for cancer tissues than for normal ones. It suggests that these two peaks can be used to diagnose diseases. The protein secondary structure of breast tissues are derived from spectra curvefit. The result suggests that the relative content ratio of a -helix to P -fold is higher in breast cancer tissues than in other diseased ones,...