Naoreqing Oral Liquid On The Mechanism Of Fever In Rabbits Heat Circulation

Fever is the familiar symptom of skull and brain post-operation, including infective fever and non-infective fever. The control of post-operational fever of skull and brain can reduce syndrome and death. The curative effect of Naoreqing(NRQ) Oral Liquid on treating fever is superior to western medicine. According to the theory or traditional Chinese medicine and based on clinical practices, Naoreqing Oral Liquid compose of huangqin ,huoxiang,niuhuang, dahuang and so on. huangqin , niuhuang and dahuang have the effect of clearing away heat and dissipate blood stasis. To study the antifebrile mechanism and clearing away heat and dissipate blood stasis effect of NRQ, the ET and EP induced fever models were established.The study consists of two parts, including the effect of clearing away heat and dissipate blood stasis , the mechanism of positive and negative adjusting in febrile rabbits.The first part: the effect of NRQ on positive and negative adjusting in febrile rabbits. In experimental research, the pureblood New-zealand rabbit, were divided randomly into nine groups: normal saline solution (NS) group, NRQ group, aspirin(ASP) group, endotoxin (ET)group, NRQ+ET group, ASP+ET group, endogenous pyrogen (EP) group, NRQ+EP group, ASP+EP group.The digital thermometer were used to register rectal temperature. The temperature curve, AT, thermal response index(TRI), of febrile rabbits were observed. At the same time, we also used the radioimmunoassay to determine the cAMP content in hypothalamus (HP) and CSF , IL-1B content in HP, AVP content in Ventral septal area(VSA).The effect of NRQ on content of positive and negative adjusting substance were observed.The results displayed as follow:1 The effect of NRQ on rectal temperature of febrile rabbits: (1)NRQ and NS group have no effects on the temperature of normal rabbits (P>0. 05) . (2)The T, TRI of ET and EP group were higher than that of NS group(P<0. 01). (3)The temperature curve, AT, TRI of NRQ+ET and NRQ+EP were separately lower than that of ET and EP group (P<0. 01). (4)NRQ and ASP therapeutic group have no obvious differences in reducing the temperature curve, AT, TRI (P>0. 05) .2 The effect of NRQ on content of positive adjusting matter in the thermoregulation nerve center of febrile rabbits: (1)The cAMP content in hypothalamus (HP) and CSF , IL-1B content in HP of NS group have no distinguished differences than that of NRQ group (P>0. 05) , but lower than that of ET and EP group (P<0. 01) . ㏕he cAMP content of NRQ+ET and NRQ+EP group were separately lower than that of ET group and EP group (P<0.01). And The cAMP content in hypothalamus (HP) and CSF , IL-1B content in HP were paralleled with the changes of temperature separately (P<0.01 or P<0. 05). (DNRQ and ASP have no obvious differences in reducing the content of cAMP in hypothalamus (HP) and CSF , IL-1B in HPof febrile rabbits(P>0. 05) .3 The effect of NRQ on content of negative adjusting matter in the thermoregulation nerve center of febrile rabbits:㏕he AVP content in VSA of NS group were lower than that of ET and EP group (P<0. 01) .(2)The AVP content of NRQ+ET and NRQ+EP group were separately lower than that of ET group and EP group (P<0. 01 or P<0. 05). (DNRQ and ASP have no obvious differences in reducing the content of AVP in VSA of febrile rabbits (P>0. 05).The second part: the effect of NRQ on clearing away heat and dissipate blood stasis of febrile rabbits. In experimental research, the pureblood New-zealand rabbit, were divided randomly into six groups: normal saline solution (NS) group, NRQ group, QKL group, ET group, NRQ+ET group, QKL+ETgroup.The measure of rectal temperature were equal to the first part. The thromboxin B2 (TXB2), 6-keto-prostaglandin F1a (6-keto-PGF1a)and endothelin content in blood plasma, and tumor necrosis factor (TNF) in blood serum were determined by radioimmunoassay. The activity of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) in blood plasma were measured by the assay of chromogenic substrate. The pathology of lung...